GC-MS analysis

Gas-chromatograph HP 6890 Series Plus, Agilent Technologies (Milano, Italia): - Column: HP5-MS (l=30 m, i.d.=0.25 mm, d.f.=0.25 μm) (Agilent Technologies); - Carrier gas: helium

- Carrier gas flux: 1 mL/min - Carrier gas pressure: 70 KPa. - Injector Temp: 200°C - Injection mode: splitless

-Temperature program : 50°C for 2 minutes (10°C/min until 200°C are reached) and then 200°C for 5 minutes (10°C/min until 260°C are reached)

Mass Spectrometer MSD 5973, Agilent Technologies: - Source Temperature: 200°C;

- Transfer Line temperature: 200°C; - Ionization: E.I. (70 eV);

-Voltage: 2200 V;

-Acquisition modality: Time scheduled monitoring; -Solvent delay: 0.5 minutes;

- Monitored ions:

from 0.5 to 5 min: m/z 91 e 92 per toluene

from 5 to 11 min: m/z 51,77,123 per nitrobenzene m/z 65,92,120 per 2-nitrotoluene m/z 65,91,137 per 3-nitrotoluene m/z 65,91,137 per 4-nitrotoluene from 11 to 28 min: m/z 76,122,168 per 1,3-dinitrobenzene

m/z 89,119,165 per 2,4-dinitrotoluene m/z 89,148,165 per 2,6-dinitrotoluene m/z 78,89,182 per 3,4-dinitrotoluene m/z 89,193,210 per 2,4,6 trinitrotoluene The Mass Spectrometer has been tuned using Perfluorotributylamine and the analytes were identified on the base of the retention time of standards solution and the MS spectra reported in NIST Library (National Institute of Standards and Technology).

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