7 Appendices
7.1 Plasmids constructed in this study
Plasmid Description
AnaCA-rbcX(TAA)N-6His-
pET28b
Plasmid encoding Anabaena sp. CA RbcXN-6His with TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAA stop codon in pET28b expression
vector (Liu et al. 2010) AnaCA-rbcX(Q5TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position Q5 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(T25TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position T25 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(H42TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position H42 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(A28TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position A28 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(T33TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position T33 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-rbcX(K54TAG,TAA)N-
6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position K54 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) AnaCA-
rbcX(L105TAG,TAA)N- 6His/Syn6301-rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 Rubisco large subunit and Anabaena sp. CA RbcXN-6His with TAG mutation at position L105 and TAA
stop codon in pET28b expression vector (Liu et al. 2010) Syn6301-rbcL(E49A)-pET11a Plasmid encoding Synechococcus sp. PCC6301
RbcL(E49A) in pET11a expression vector (Bracher et al. 2011)
Syn6301-rbcL(E49C)-pET11a Plasmid encoding Synechococcus sp. PCC6301 RbcL(E49C) in pET11a expression vector (Bracher et al. 2011)
Syn6301-rbcL(A53H)-pET11a Plasmid encoding Synechococcus sp. PCC6301 RbcL(A53H) in pET11a expression vector (Bracher et al. 2011)
Syn6301-rbcL(A53T)-pET11a Plasmid encoding Synechococcus sp. PCC6301 RbcL(A53T) in pET11a expression vector
Plasmid Description Syn6301-rbcL(A126T)-
pET11a
Plasmid encoding Synechococcus sp. PCC6301 RbcL(A126T) in pET11a expression vector
Syn6301-rbcL(A126Y)- pET11a
Plasmid encoding Synechococcus sp. PCC6301 RbcL(A126Y) in pET11a expression vector (Bracher et al. 2011)
AnaCA-rbcXN-6His/Syn6301-
rbcL-pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcXN- 6His and Synechococcus sp. PCC6301 RbcL in pET28b
expression vector (Bracher et al. 2011) AnaCA-rbcXN-6His/Syn6301-
rbcL(E49C)-pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcXN- 6His and Synechococcus sp. PCC6301 RbcL(E49C) in
pET28b expression vector (Bracher et al. 2011) AnaCA-rbcX(T33C)N-
6His/Syn6301-rbcL(E49C)-
pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcX(T33C)N-6His and Synechococcus sp. PCC6301
RbcL(E49C) in pET28b expression vector (Bracher et al. 2011)
AnaCA-rbcX(E83C)N- 6His/Syn6301-rbcL(E49C)-
pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcX(E83C)N-6His and Synechococcus sp. PCC6301
RbcL(E49C) in pET28b expression vector (Bracher et al. 2011)
Syn6301-rbcX(T51C)N-6His-
pET28b
Plasmid encoding Synechococcus sp. PCC6301 RbcX(T51C)N-6His in pET28b expression vector
Syn6301-rbcX(D102C)N-6His-
pET28b
Plasmid encoding Synechococcus sp. PCC6301 RbcX(D102C)N-6His in pET28b expression vector
Syn6301-rbcXN-6His/Syn6301-
rbcL-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-6His and Synechococcus sp. PCC6301
RbcL in pET28b expression vector Syn6301-rbcXN-6His/Syn6301-
rbcL(E49C)-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-6His and Synechococcus sp. PCC6301
RbcL(E49C) in pET28b expression vector Syn6301-rbcX(T51C)N-
6His/Syn6301-rbcL(E49C)-
pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcX(T51C)N-6His and Synechococcus sp.
PCC6301 RbcL(E49C) in pET28b expression vector Syn6301-rbcX(D102C)N-
6His/Syn6301-rbcL(E49C)-
pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcX(D102C)N-6His and Synechococcus sp.
PCC6301 RbcL(E49C) in pET28b expression vector Syn6301-rbcXN-6His/Syn6301-
rbcL(F342I)-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-6His and Synechococcus sp. PCC6301
RbcL(F342I) in pET28b expression vector (Bracher et al. 2011)
Syn6301-rbcXN-6His/Syn6301-
rbcL(E49C,F342I)-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-6His and Synechococcus sp. PCC6301
RbcL(E49C,F342I) in pET28b expression vector (Bracher et al. 2011)
Syn6301-rbcX(T51C)N- 6His/Syn6301-
rbcL(E49C,F342I)-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcX(T51C)N-6His and Synechococcus sp.
PCC6301 RbcL(E49C,F342I) in pET28b expression vector (Bracher et al. 2011)
Syn6301-rbcX(D102C)N- 6His/Syn6301-
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcX(D102C)N-6His and Synechococcus sp.
Plasmid Description
rbcL(E49C,F342I)-pET28b PCC6301 RbcL(E49C,F342I) in pET28b expression vector (Bracher et al. 2011)
Syn7002-rbcL
(E48C)/Syn7002-rbcXN-6His-
pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcXN-6His and RbcL(E48C) in pET28b
expression vector Syn7002-rbcL
(E48C)/Syn7002-
rbcX(T33C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(T33C)N-6His and RbcL(E48C) in pET28b
expression vector Syn7002-rbcL
(E48C)/Syn7002-
rbcX(E84C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(E84C)N-6His and RbcL(E48C) in pET28b
expression vector Syn7002-rbcL (E48C,F341I)
–pET11a
Plasmid encoding Synechococcus sp. PCC7002 RbcL(E48C,F341I) in pET11a expression vector
Syn7002-rbcL
(F341I)/Syn7002-rbcXN-6His-
pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcXN-6His and RbcL(F341I) in pET28b
expression vector Syn7002-rbcL
(E48C,F341I)/Syn7002-
rbcX(T33C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(T33C)N-6His and RbcL(E48C,F341I) in
pET28b expression vector Syn7002-rbcL
(E48C,F341I)/Syn7002-
rbcX(E84C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(E84C)N-6His and RbcL(E48C,F341I) in
pET28b expression vector Syn7002-rbcL
(F341I)/Syn7002-
rbcX(T33C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(T33C)N-6His and RbcL(F341I) in pET28b
expression vector Syn7002-rbcL
(F341I)/Syn7002-
rbcX(E84C)N-6His-pET28b
Coexpression plasmid encoding Synechococcus sp. PCC7002 RbcX(E84C)N-6His and RbcL(F341I) in pET28b
expression vector Syn7002-rbcX(G42H,E43N)N-
6His-pET28b
Plasmid encoding Synechococcus sp. PCC7002 RbcX(G42H,E43N)N-6His in pET28b expression vector
Syn6301-rbcL(E106Q)- pET11a
Plasmid encoding Synechococcus sp. PCC6301 RbcL(E106Q) in pET11a expression vector (Bracher et al. 2011)
AnaCA-rbcXN-6His/Syn6301-
rbcL(E106Q)-pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcXN- 6His and Synechococcus sp. PCC6301 RbcL(E106Q) in
pET28b expression vector (Bracher et al. 2011) Syn6301-rbcL(R212S)-
pET28b
Plasmid encoding Synechococcus sp. 6301 RbcL(R212S) in pET11a expression vector (Bracher et al. 2011)
AnaCA-rbcXN-6His/Syn6301-
rbcL(R212S)-pET28b
Coexpression plasmid encoding Anabaena sp. CA RbcXN- 6His and Synechococcus sp. PCC6301 RbcL(R212S) in
pET28b expression vector (Bracher et al. 2011) Syn6301-rbcXN-FLAG/Syn6301-
rbcL-pET11a
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-FLAG and Synechococcus sp. PCC6301
RbcL in pET11a expression vector (Bracher et al. 2011) Syn6301-rbcXN-FLAG/Syn6301-
rbcL(W67A,L70G,L71A)- pET11a
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-FLAG and Synechococcus sp. PCC6301
RbcL(W67A,L70G,L71A) in pET11a expression vector (Bracher et al. 2011)
Plasmid Description rbcL(W67A,L70G,L71A)-
pET11a
RbcL(W67A,L70G,L71A) in pET11a expression vector (Bracher et al. 2011)
Syn6301-rbcLΔN(1-11)- pET11a
Plasmid encoding Synechococcus sp. PCC6301 RbcLΔN(1-11) in pET11a expression vector
Syn6301-rbcXN-FLAG/Syn6301-
rbcLΔN(1-11)-pET11a
Coexpression plasmid encoding Synechococcus sp. PCC6301 RbcXN-FLAG and Synechococcus sp. PCC6301
RbcLΔN(1-11) in pET11a expression vector Syn6301-rbcLΔN(1-5)-
pET11a
Plasmid encoding Synechococcus sp. PCC6301 RbcLΔN(1-5) in pET11a expression vector
Syn6301-rbcS(L60A,F63A)- pET11a
Plasmid encoding Synechococcus sp PCC6301 RbcS(L60A,F63A) in pET11a expression vector
Ana7120-groEL1-pET11a Plasmid encoding Anabaena sp. PCC7120 GroEL 1 in pET11a expression vector
Ana7120-groEL2-pET11a Plasmid encoding Anabaena sp. PCC7120 GroEL 2 in pET11a expression vector
Ana7120-groES-pET11a Plasmid encoding Anabaena sp. PCC7120 GroES in pET11a expression vector
Ana7120-groEL2- pCOLAduet-1
Plasmid encoding Anabaena sp. PCC7120 GroEL 2 in pCOLAduet-1 expression vector
Syn6301-groEL1-pET11a Plasmid encoding Synechococcus sp. PCC6301 GroEL 1 in pET11a expression vector
Syn6301-groES-pET11a Plasmid encoding Synechococcus sp. PCC6301 GroES in pET11a expression vector
Table 7-1 Plasmids designed in this study.