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Exploitation of Feedback in Enzyme-catalysed Reactions

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Figure

Figure 1. (a) Bell-shaped rate-pH curve characteristic of enzyme catalyzed reactions and corresponding protonation equilibria of the enzyme (b) Change in pH in a closed reactor for the urea-urease reaction
Figure 2. (a) Model scheme for pH driven feedback in an open  constant supply of substrate and acid from the surroundings
Figure 3. (a) Cross-shaped phase diagram with pH driven  simulations (c) pH oscillations in the experiment
Figure 4. Design of enzyme reaction networks that are autocatalytic in acid/base; (b) compartmentalisation of the reaction by preparation of enzyme-loaded microparticles; (c) generation of a switch to low/high pH states, a pH gradient or periodic pH pulses in response to a chemical signal (d) characterisation of collective behaviour such as a synchronised switch

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