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Measuring proteins in H2O with 2D-IR spectroscopy

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Figure 1: (a) The IR absorption spectra of Dγ-globulins,subtraction of the Hof water and the protein amide I band at 1650 cm2O (red) and H2O (blue)
Figure 2. (a) IR absorption and (b) 2D-IR spectrum of pure serum in the amide I region
Figure 3. IR absorption spectra of (a) serum (b) serum albumin (c) (f) γ-globulins. 2D-IR spectra of (d) serum (e) serum albumin γ-globulins
Figure 5: AGR of serum samples from 2D-IR spectroscopy. a&b) AGR obtained using the 2D-IR diagonal method
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