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Second-Generation Rabies Virus-Based Vaccine Vectors Expressing Human Immunodeficiency Virus Type 1 Gag Have Greatly Reduced Pathogenicity but Are Highly Immunogenic

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Figure

FIG. 1. Construction of different recombinant vaccine vectors expressing HIV-1 Gag. At the top are two RV vaccine strain-based vectorscontaining an additional transcription stop-start signal flanked by two unique restriction sites between the G and L genes (SPBN) or the N and
FIG. 2. Western blot analysis of recombinant vaccine vectors. BSR cells were infected with SPBN, SPBN-Gag, SPBN-333-Gag, SPBN-�Gag, SPBN-333-blotting with antibodies specific for RV RNP (A), RV G (B), or HIV-1 p24 (C)
FIG. 4. New RV-based vaccine vectors are safe even after i.c. in-oculation in mice. Six- to 8-week-old Swiss-Webster mice were inocu-
FIG. 6. Recombinant vaccine vehicles induce similar humoral andcellular immune responses in immunized mice

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