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Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling

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Figure 1 Oestrogenic effects on cancer stem cellscontrol and CSC assays were performed in ER positive cells (primary, MCF7, T47D and BT474) and ER negative cells (231)
Figure 2 Knock-down or inhibition of ER signalling blocks the effect of 17b-estradiol
Figure 3 Breast cancer stem cells have low expression of ERand cell lines (MCF7 and T47D) were collected for protein and RNA
Figure 4 Systemic oestrogen signalling is mediated by EGFR and Notch. (A) Representative Western blot showing expression of cleaved(active) Notch1 (N1-ICD) following culture ± 1 nM 17b-estradiol ± 10 μM GSI
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