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Effects of Different RNAs and Components of the Cell-Free System on In Vitro Synthesis of Sindbis Viral Proteins

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Figure

TABLE 1. Purification of Sindbis mRNA frominfected BHK cells
FIG.1.ontoSpincoRNA,tractedsamplecentrifuged Rate-zonal centrifugation of RNAex- fromvirions.Phenol-chloroform-extracted after precipitation with ethanol, was layereda10to 20%sucrose gradient(wt/wt) and for 12 h at 20,000 rpm at 15 C in the rotor SW27
FIG. 3.described(see SDS-polyacrylamide gel patterns of proteins formed in vitro by different RNAs
FIG. 4.proteinsfromprofilescribedinfractionviralsucrose-ureaRNA;subunits;treatedin(A)ts-2in the a a Rate-zonal centrifugation ofprotein formed reticulocyte system
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