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Characterisation of P glycoprotein 9 1 in Haemonchus contortus

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Figure

Table 1 Primers for qRT-PCR (quantitative Real-Time-PCR) onscorfaHaemonchus contortus P-glycoprotein-9.1/epithelial-like pig (Sus) kidney cell (Hco-Pgp-9.1/LLC-PK1) transfected cells
Fig. 1 qRT-PCR onlevel present in the transfected pig host cells corresponding to cellculture passages 2 and 3
Fig. 2 Western blot using anti-from(Chloramphenicol acetyl transferase)/LLC-PK1 membranes (control cells).Each lane was loaded with 5contortusHco-PGP-9.1 antibody
Fig. 4 Functional transport assay using the fluorophore probeRhodamine 123 (Rho123). Fluorescence in control cells (untransfectedLLC-PK1, and CAT transfectants); cells over expressing the mouse PGP(MDR1A– positive control) and the nematode transporter cell
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