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Influenza B viruses with site-specific mutations introduced into the HA gene.

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Figure

FIG. 1. (a) Plasmid pT7NSBCAT has been previously described as plasmid IVBCAT (16). The construction of plasmid pT3HABCAT was essentially the same asthat for pT7NSBCAT (16) and utilized PCR cloning techniques with appropriate primers
Fig. 3b. When model RNAs harboring these deletions andmutations were transfected, all of the replicons produced com-parable levels of CAT activity which could be passaged to a
FIG. 3. (a) Sequences of the 5�by capital letters, and deviations are indicated by lowercase letters or by dashes for deletions
FIG. 4. Primer 1702 was end labelled with [32as a pure product. The products of the two reactions for each vRNA were mixedand loaded together onto the gel

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