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Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9

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Figure

Fig. 1 Plasmid map of pL6cs-hDHFR-ck2using an in-fusion method. The plasmid map of pL6cs-hDHFR-ck2ck2α
Fig. 3 Genomic DNA PCR to confirm the tagging. Using genomic DNA as the template and specific primers of each gene (P33/P34 for ck2β1,P1/P2 for ck2α, P35/P36 for stk), the products obtained by PCR were separated on an agarose gel
Fig. 4 Western blot to confirm the tagging. The tags added to CK2the successful tagging

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