Nucleotide sequence and structural determinants of specific binding of coat protein or coat protein peptides to the 3' untranslated region of alfalfa mosaic virus RNA 4.

To study the initial interaction between this 3 ⴕ UTR and cellular and viral proteins, mobility shift assays were performed, and four ribonucleoprotein complexes (I through IV)

RNA probes carrying the sequence GCGC in place of the central four contiguous uridines of the 8-nucleotide U-rich motif fail to bind host protein at detectable levels, and the

assembly, and spread of infection. We found that while coat protein accumulation was only moderately affected, RNA packaging in vivo was completely abolished by the loss of

length RNA; (ii) coat protein (P3; molecular weight, 29,000) induced by a sucrose gradient fraction containing predominantly RNAs in the.. molecular-weight range of 0.3 x 106 to 0.4

The 3’ terminal region of about 1.7 kb comprising a part of the nuclear inclusion b (NIb) gene, complete coat protein (CP) gene and the 3’ untranslated region (3’UTR) of seven

Assays for protection of RB69 gp43 from clostripain by operator RNA (Fig. 4B) suggest that the interaction with specific RNA affects access of the protease to sites in the