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Ribosomal Protein P0 Promotes Potato Virus A Infection and Functions in Viral Translation Together with VPg and eIF(iso)4E

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Figure

FIG 1 Ribosomal P0 is a component of membrane-associated viral RNPs. (A)Western blotting showing the presence of P proteins in the RNP complexesobtained by affinity purification, using Strep III-tagged PVA RdRp (RdRpSIII)and Strep III-tagged VPg (VPgSIII), f
FIG 3 Ribosomal P0 promotes PVA infection. (A) P0 was silenced by expressing an RNA hairpin (hp) that targets P0 transcripts (hpP0), followed by Western(B) Exogenous P0 was expressed, and P0 levels were analyzed by Western blotting as described above for p
FIG 4 Ribosomal P proteins are important for PVA infection. (A) RLUC activity of wt PVA was determined at 3, 6, and 9 DAI during silencing of P proteins by
FIG 6 P0 can increase viral translation. (A) Exogenous P0 was coexpressedwith PVAand 0.5 for GUS and P0.�GDD and FLUC, and luciferase activity and RNA amounts wereassessed at 3 DAI
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