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Akt and phospholipase Cγ are involved in the regulation of growth and migration of MDA MB 468 breast cancer and SW480 colon cancer cells when cultured with diabetogenic levels of glucose and insulin

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Academic year: 2020

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Figure

Figure 1 Western blot analysis of Akt and PLCγ expression.Western blot analysis was performed to evaluate Akt and PLCγexpression and shows that Akt expression was up-regulated in bothcell lines when additional glucose was available (A)
Figure 2 Tumor cell proliferation with high glucose and insulin.***MDA-MB-468 breast cancer and SW480 colon cancer cellssignificantly increased proliferation rates after addition of insulin(100 ng/ml) and doubling glucose concentrations
Figure 3 Tumor cell migration with high glucose and insulin. MDA-MB-468 breast cancer and SW480 colon cancer cells reduced migratoryactivity (MA) after addition of Akt-inhibitor A6730 and PLCγ-inhibitor U73122 when compared to cells stimulated with increas

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