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Down regulation of poliovirus receptor RNA in HeLa cells resistant to poliovirus infection.

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Academic year: 2019

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Figure

FIG. 2.transferredgene.fromcells.2H2,cDNAuncloned Northern blot analysis of total cell RNA from SOFIA Samples (10 ,ug) of total cellular RNA from HeLa cells (H), SOFIA cells (S), and several single-cell clones derived SOFIA cells (1C3, 1C6, 1E7, 2G5, 2D5, 2D11, 2E6, 2F3, 2G9, and 3E5) were fractionated in a 1% agarose-formaldehyde gel,to nitrocellulose, and hybridized witha 32P-labeled probe representing almost all the coding sequence of the PVR Positions of 28S and 18S rRNA markers are indicated.
FIG. 3.ofaHeLapaper.with32P-labeledprobe.Positions 1% SOFIA Northern blot analysis of poly(A)+ RNA from clone 2G9 cells
FIG. 5.andweremonolayersintensifying Induction of PVR at the cell surface of SOFIA cells treated with 5-azaC
FIG.was performed asesSOFIA were resistant tor other clones were.3-kb PVR cells R mRNA treatedwithclones'NA fromdescribed single-cellpoliovirusindicated.susceptible 6

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