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Minute Virus of Mice NS1 Interacts with the SMN Protein, and They Colocalize in Novel Nuclear Bodies Induced by Parvovirus Infection

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Figure

FIG. 1. SMN and NS1 interact directly both in vitro and in vivo. (A) Western blot analysis of recombinant polyhistidine (six-His-)-tagged humanSMN captured by GST-tagged NS1 immobilized on GST resin
FIG. 2. Colocalization of SMN, p80 coilin, SIP1, and NS1 in transfected A9TRITC [red]), SIP1 (primary antibody: MANSIP1A; secondary antibody: anti-mouse TRITC conjugate [red]), p80 coilin (primary antibody: 5P10monoclonal antibody; secondary: anti-mouse TR
FIG. 3. SMN, p80 coilin, and NS1 colocalize in SAABs in MVM-infected A9regions of interest magnified by an additional factor of 2
FIG. 4. SAAB formation is neither a consequence of the blocking process nor restricted to murine cell lines
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