Molekulare Analyse der Traubenarchitektur (Verbundvorhaben)

Dieses Dokument steht unter www.orgprints.org/32365/ zum Herunterladen zur Verfügung.

Molecular analysis of grapevine cluster architecture

FKZ: 11NA056 und FKZ: 11NA093

Projektkoordination:

Julius-Kühn Institut - Bundesforschungsinstitut für Kulturpflanzen,

Institut für Rebenzüchtung Geilweilerhof

Geilweilerhof, 76833 Siebeldingen

Tel.:

+49 6345 41-0

Fax:

+49 6345 41-179

E-Mail: zr@julius-kuehn.de

Internet: www.julius-kuehn.de/zr/

Autoren:

Zyprian, Eva; Theres, Klaus

Molekulare Analyse der Traubenarchitektur

Gefördert durch das Bundesministerium für Ernährung und Landwirtschaft aufgrund eines Beschlusses

des Deutschen Bundestages im Rahmen des Bundesprogramms Ökologischer Landbau und andere

Formen nachhaltiger Landwirtschaft.

/͘^ĐŚůƵƐƐďĞƌŝĐŚƚ

DdͲDŽůĞŬƵůĂƌĞŶĂůLJƐĞĚĞƌdƌĂƵďĞŶĂƌĐŚŝƚĞŬƚƵƌ

!"#$%&'()(*+

,

.+ /

/%$')00"+

+-12!34

567-#)8,&9':;9))

#<=17/56 >;

3<=17

1 %?3@A> &%9&)#B6

+-12C

567#)8,&B0

@;+-&&#&(D0&&B#&$ -&&#&(D0&&B#&$

DdͲDŽůĞŬƵůĂƌĞŶĂůLJƐĞĚĞƌdƌĂƵďĞŶĂƌĐŚŝƚĞŬƚƵƌ

!34%.+ /%$')00"+ %

34EF7

C%<=171 %9&)#B6

E

1F7 4 /

7= <7 @7+ 7 + 2 4

?7 7 % 57. %

6G C+7+ / 2 7

1 *C@ ,4 7

"+ 7 = 7 /4 C+7

>

% !7 , <7

@7+ 7 " + "+ 7 7 : 7

< % @7+ 7 7 + <

" C+7

"4

HIJKHLMLNLHOPQPRSMLTIUQNIJHKPVIMLMJWQVIJVKMI

CF+4 +7 =

3 !=34

% +4

/+X3*C@

Y1 8Z 4 4

4 3

Y1

Z 4 "3

4 3 [

/ŶŚĂůƚƐǀĞƌnjĞŝĐŚŶŝƐ

,+7 3 ";

C+3 ";

,++ 3 ";

! "

/ ;+ "

# "

01 ,+1F7 "

#>"1F7+ "#

0<< "(

0< "(

0#< "(

0#14 "(

0##<7 ,4 "'

0##*C@,4 "'

0###/= "'

0##0.8, 28,"X 8= X 8/" "$

0##( C3\]^_`abcd`def^g`^h^ "$

0#0.777 "$

(,2 ! + ")

(i @ 5 ")

(#i 3C7@7+ 7 ")

(0i 3" ")

((14 i "+ 7 "B

(9 3 .8,"X 28,"X "B

('>/=4- "&

($! +*C@,41/5/,($(#=j;+Z "

() /= "#

927! + "0

', +38;+7! + "9

$/ +

%:5 "'

) "$

B@3 "B

&k+;6 % "#&

, "+ ! 7+ "##

ďŬƺƌnjƵŶŐƐǀĞƌnjĞŝĐŚŶŝƐ

,C 4,4C

?: !7 >+

%?

28, .8,37+728,

< <

28, 2=4+7

5 !5

28,"X 28,"X

/

@l2 %" 73*C@

.8, .8,

8/" = X

*C@ *3

.8, .+7

.8,"X .+7"X

"81 4

dĂďĞůůĞŶǀĞƌnjĞŝĐŚŶŝƐ

dĂďĞůůĞϭ

^͘ϲ

mďĞƌƐŝĐŚƚƵŶĚWƌŽďĞŶƵŵĨĂŶŐĚĞƌĂƚĞŶƉƌŽ'ĞŶŽƚLJƉnjƵhŶƚĞƌŵĞƌŬŵĂůĞŶĚĞƌ

dƌĂƵďĞŶĂƌĐŚŝƚĞŬƚƵƌ

ďďŝůĚƵŶŐƐǀĞƌnjĞŝĐŚŶŝƐ

ďďŝůĚƵŶŐϭ

^͘ϵ

ƌĞŝŶƚǁŝĐŬůƵŶŐƐƐƚĂĚŝĞŶǀŽŶ^ƉćƚďƵƌŐƵŶĚĞƌŝŶĨůŽƌĞƐnjĞŶnjĞŶ͕ĚŝĞĨƺƌĚŝĞZEͲ^ĞƋŶĂůLJƐĞ

ǀĞƌǁĞŶĚĞƚǁƵƌĚĞŶ

ďďŝůĚƵŶŐϮ

^͘ϭϬ

DĂƉƉŝŶŐƉƌŽĨŝůĞ;ƌŽƐĂͿĚĞƌǀŝĞƌ^ƉćƚďƵƌŐƵŶĚĞƌŬůŽŶĞŐĞŐĞŶĚŝĞ<ŽĚŝĞƌƵŶŐƐƐĞƋƵĞŶnjĞŝŶĞƐ

<ĂŶĚŝĚĂƚĞŶŐĞŶĞƐ;ŐĞůďͿ͘

ďďŝůĚƵŶŐϯ

^͘ϭϭ

WŽƐŝƚŝŽŶĚĞƌYd>ƐĨƺƌhŶƚĞƌŵĞƌŬŵĂůĞĚĞƌdƌĂƵďĞŶĂƌĐŚŝƚĞŬƚƵƌĂƵĨĚĞŶϭϵ<ŽƉƉůƵŶŐƐŐƌƵƉƉĞŶ

ĚĞƌŐĞŶĞƚŝƐĐŚĞŶ<ĂƌƚĞĂƵƐĚĞƌ<ƌĞƵnjƵŶŐƐƉŽƉƵůĂƚŝŽŶ'&͘'ͲϰϳͲϰϮdžഹsŝůůĂƌĚďůĂŶĐ͛͘

ϭ͘ŝŶĨƺŚƌƵŶŐ

ϭ͘ϭ'ĞŐĞŶƐƚĂŶĚĚĞƐsŽƌŚĂďĞŶƐ

mbe]ne`d%! /% " >++

6 op >+ G 8

>7 . "

+7 2+ 7 mbe]ne`d /

;+5 %!31!

7F4717

47 57 6 G + @7+ 7 5

1F7 !+ 7/ , + 3

<7 @7+ 7 .+ < <7 7 7 <7

76 .+ % >+

75

ϭ͘ϮŝĞůƐĞƚnjƵŶŐ

mbe]ne`d q`hr]r^ C- mbe]nbe`h`^ stqurg`^h^ 1 7 @+%>73+#&&>#&&$ 5mv

q`hr]r^ 7 . +7 .+ 7

. 3 + 2 ,+7 l+ 6 7

C+7F47" % mvq`hr]r^

2 14 87 + <7 C+7

/5/,($(# YZ = Y"43Z

G + Y; +Z /+ %

;+ 3 7 C+7 3 2 = 7+

C+ Y?Z5% . @7+ 7 =

7 77 /4 1

/=4 3 + 7 77

Y18Z"+ 5%/%

714 3 <7

7 % @7+ 772 7 57 3

" , <," D<7 "7 8.+

.7 " + .7

1% >+

ϭ͘ϯWůĂŶƵŶŐƵŶĚďůĂƵĨĚĞƐWƌŽũĞŬƚĞƐ

8; #&0#&(%"+ wxyzIQH{IQHIMWR

#&9 #&' 4 ?7 3

; i7C+732

3 /4 7 5 #&9 #&'

5+777C+ 3%

>47/42wxy#&9%#&'#&$

">+ 7F1+

375 Y18Z/4 2.8,

Y18Z .8,"X <7 + / != 3

%9 @ ++ 57

7 % C7 % %

7 +4;+ +7 , |yx}HN

l 6 C+7 <

2l >+

3 .8,"X" "X 28,*C@

@7+ 77 wxyzIQH{IQHIMWR +

C+71 //,($(#= Y;+Z 3 +

7 4 #&' #&0 + #&$ + 4 2

+ % 7 !=% +

@7+ 7 % +

/= ; 7 77 /4 2

"X4 |y x}HN "X4

% . 3 < ! /%

i 33 % + 1

C \]^_`abcd`d ef^g`^h^ 7 ;

! +!34j18Z:

.7777

Ϯ͘tŝƐƐĞŶƐĐŚĂĨƚůŝĐŚĞƌ^ƚĂŶĚnjƵWƌŽũĞŬƚďĞŐŝŶŶ

!+7%mbe]ne`d;3"%3C

/% G3+, 327

BBB 2 ,4 7 5 7% C o< p

. 5 C31% #&&B~

:% #&&) 2 4 @+ 3 mv q`hr]r^ 7

+ % + ; 3 > 3 3 +

" / .+ 5

7 2 <4 ? >% + ,

: 3% ? 2 ! 3

@%1 " +?BBB 2

++<7; 2

+6 > 5 #&&$ 2 7 7

7 3 mv q`hr]r^ + .+ 7 C 2

l+ C+ BB9~ ?+ #&9 @7

C+77 7;<% BB

,+7 ; 3 +X + <72

l .+ > l; 7 27 #&(

-[[3[[[ 3[3

33# 7 3 C+

+."@7+ 72<7@7+ 77

< / + Y? Z

C 73 < €‚ƒ„\ / +7%

7 3 77 5 #&& ! =

i 77+ .+ Y. Z Y?4Z ;

7+ " Y"Z Y!=Z "373 #&&0 +%

i 77 7 5

5 . 37 = 2 i

+17 +3G

! 7 !

" :+*C@ ,4 3<7 C+7

C+ Y.+4 "Z Y"Z + .

, 8 : @7+ 7

? #&( " ,4 3 *C@.

<7 @7+ 7 + 7 7 57

?9%)%B%(%$)

5 3 //,($(# = j; +Z% + <7

C+7 / i %

5 3+ 7; 4#&'

ϯ͘DĂƚĞƌŝĂůƵŶĚDĞƚŚŽĚĞŶ

ϯ͘ϭDĂƚĞƌŝĂů

…† /5/,($(#=Y;+Z

2953 + l;

77‡7+B‡77 "+

<7@7+ 7 ,""+ F1

/4;

ˆ†Y18Z7+ 77C+7

2;73+l;#&( 2Y1

8Z/

-•

->!$%>!#(#%>!<%/)'%5.#@%5.0@

•

-/#&0%5.)&

•

C+-57?%57?%!8C,;$$$%/)

2 Y18Z" F +,

-•

•

•

2! ++; +i

"i Y#9,,Z21%)+#%#‰

1 3 + ! F + .+67

; ; 2 1 o +p% 76

3F

Š† 2 " Y?Z% C+% Y, @3AZ =

Y6 > Z" = 7+ 14%14

Y6 > Z,.+"/

ܠ;l;+.@7+

‡ 7%Yi3.Z $‡%Y"3 +Z

0‡ 7%Y1;Z B‡%Y1<Z

9‡ %Y"3/Z

2 ; + 1 .

/

ϯ͘ϮDĞƚŚŽĚĞŶ

ŒŽ|WNVOTQPQIMKNS

#&0 + #&$ C+ <

3 2+14 1, % %? 2

-•

@ 5+, % ? #&'l;

.+ 2 ! + 3 /4[F

+ + C+ / ‡' ,

F & 3 1 6G + 7

. + i ;F < 3 ~ +

? 1 2 <

#&9 #&' F 3 !7 ?:90 /

+ +?:$B!C+

•

!7 ?:)B@

•

•

%@1

•

,;

•

˜MLK’INLMJWQVI—VKM Ž™Œ Nš› Ž™œ NšŒ Ž™ NšŽ Ž™› Nš› Ž™ž Nš› …’—ŸM•KNS IM—”LH         ¡¢ ¡££¤£¥¦¥§¦¨©    

¡ª ¡££¤£¥«£¬­®¨¯°«±  

²³ ´¤¦µ¶£¥·¸ ©µ¹£¥«£¯¦µ®¨¯°¹ ©±

²ª ´¤¦µ¶£¥«£¬­®¨¯

º»¼ º½¥«£¾£¿»À¼¥¯£¤¥¸¾­µ¹¿¾£¤Á¦®¨­¿ °¹¹±

º»º º½¥«£¾£¤»Àº¦¯£¤¦©Â³£¤§¬£­«µ¥«

ºÃ¼ º½¥«£¾£¿ÃÀ¼¥¯£¤¥¸¾­µ¹¿¾£¤Á¦®¨­¿  

ºÃº º½¥«£¾£¤ÃÀº¦¯£¤¦©Â³£¤§¬£­«µ¥«

ºÄ¼ º½¥«£¾£¿ÄÀ¼¥¯£¤¥¸¾­µ¹¿¾£¤Á¦®¨­¿  

ºÄº º½¥«£¾£¤ÄÀº¦¯£¤¦©Â³£¤§¬£­«µ¥«  

ºÅº º½¥«£¾£¤ÅÀº¦¯£¤¦©Â³£¤§¬£­«µ¥«  

ºÆº º½¥«£¾£¤ÆÀº¦¯£¤¦©Â³£¤§¬£­«µ¥«

Ç¡³ È­¥§£©Â¡££¤£¥·¸©µ¹£¥

ÉÈ

Mostgewicht °Oechsle

ɼ³ÃÊŠɼ³ÃÊÅ¡¸¥­¯µ¤¥ ¸¯£

ËÈÌ Ë£¾­®£©©½ ¥«£

˺ ͯ­£©ÂÎË£¾ µ¥Ï£©Ðº½¥«£

ÁÌ Á¦®¨­¿¾ µ¤®¨¹£¿¿£¤

Áº Á¦®¨­¿ ©½¥«£

Áª Á¦®¨­¿«£¬­® ¨¯

ͺ Í®¨µ ©¯£¤¯¤¦µ¶£¥©½¥«£

´¡³²¡ Ñ£¿¦¹¯Â¡££¤£¥·¸©µ¹£¥·­¿µ£© ©«£Ò¤ÓÔ¯  

´¡³Ì Ñ£¿¦¹¯Â¡££¤£¥·¸©µ¹£¥«£¯¦µ®¨ ¯

´ºº Ñ£¿¦¹¯©½¥«£º¦¯£¤¦©Â³£¤§¬£­«µ¥«

ŒŽŽ HI—KHLMIKN“SINIVQPJWI…NLHOPIN

ŒŽŽÕ˜ÖׅNLHOPIN

< < , *C@ *3 C @ ,4 +

1/5/,($(#=j;+Z4#&'

< 1 <,1 *C@ '& 3 < " 2

+ 1 @l2 "% + !=

*C@ 7 % + <,1 *C@'

" 733&%&9&&&1 3lF#&&B

ŒŽŽŽzINI TMIPPQNPPVK“QIN

< , % % ? 2 /= 3 , .8,

!=7 28, "4 1+% 7

"7 : .

.+ 18(&&#(% % #&&$ l @ 3 )#$ 87

" 3 9)'#Ø? + 2 ,7 /6G 3 9&9&

. ! 7 @ 3

C7+ 73 ;7 3 47

8; C4<7:2 /=

0& 47 C17- /!B'ÙB' 5,"C

3# 23<7B'B'24,4?6 !=B'28,

1+ B' 1 + <= 3 B#' +

.7+7, 3/=<7

:2"4 " " 35 .C1?.,4

"(0

ŒŽŽŒÚۅ×KN“S‹Û…ÜIÝKIN•QIMKNSÞÛI VSINIMLVQNPIÝKINJQNSÞÛz܆†

5.8,"X 1+3>!$%/)'%57?57?

" "+ 3 !7 F +

>

-"-"+#&(#&9

"#-?:$73+#&9

"0-. +3"?:9$73+ #&9

2 .8, "ß 1C .8, " , = 5 28,

"X 3%!=7 X/l@2128,

< 1X+ , 8/""X <=17/ 6

2 , 2 " ?@? /

>7+

ŒŽŽœxHNQIMKNSKN“˜MLNPRM”LVQNUNàáâãäåæçèäèéêâëäâìâ

5/77ímîïíîîð„ñ13%+//

4 @ % #&0 \]^_`abcd`d ?& 1

p52p<? ò%BB)

ŒŽŒÚLPVIMIHI—VMNIN”Q—MP—TLKRNLW”IN

.777 77 7 < 2

@ 31 "3"%

#&#

ϰ͘ƵƐĨƺŚƌůŝĐŚĞĂƌƐƚĞůůƵŶŐĚĞƌǁŝĐŚƚŝŐƐƚĞŶƌŐĞďŶŝƐƐĞ

ϰ͘ϭhŶƚĞƌƐƵĐŚƵŶŐĞŶnjƵŵ>ćŶŐĞŶnjƵǁĂĐŚƐĚĞƐ&ƌƵĐŚƚƐƚĂŶĚĞƐ

,.+ zIQH{IQHIMWR7+77Y18Z

7- ><$% /)'% /#&0~ 77- 57 ?% 57 ?

@ @ C++ , '

+ C+ Y1 8Z + !7

?:0( < < 6 + ?:$$ , , 2 < 3

#&9#&' +PQSNQRQ—LNVPJWNIHHIMIN֐NSIN•K{LJWPQN“INHJ—IM’IIMQSINxHNIN K” “IN óIQVTKN—V “IM ˆHŸVI ވˆŠô›† 2 - 2

47 7 77 Y1 8Z 7

, /=" ,""+

< + >

/5/,($(#Y;+Z3 2@ 6G+

7 3 1% + . F ;F

#&([#&9 % : #&' #&9% 3

6G . ; 7 7 @

>ܔQV’IPVVQSVIPQJW“IM•KUM’IPJWMQI’INIˆIRKN“ “QRRIMINVQIHHINõLJWPVK”P LNKNL’W NSQSI”|RHLN•IN”LVIMQLH

ϰ͘ϮhŶƚĞƌƐƵĐŚƵŶŐĞŶǀŽŶdĞŝůĂƐƉĞŬƚĞŶĚĞƌ>ŽĐŬĞƌďĞĞƌŝŐŬĞŝƚ

7 @ ?:)B wxy 3 # Y1 8Z 3

" F &C+ iC+7 3

2 !3 3 i7 C+7 3 C+ +

< 6G 7i#Y1 8Z o1p C

+ <7 ÚLJWQPHNSIö |I“QJIHHNSI KN“ ÷QN•IH’IIMINUHK”IN PQSNQRQ—LNVI

–NVIMPJWQI“I 7 77Y1 8Z 2 + "

;F

29587 /5/,($(# Y;+Z3%

i7C+7 132l;

27 #&( 52 +

++ +C+ ++

2 ! +3 i7 C+7

l;27G 3*C@

ϰ͘ϯhŶƚĞƌƐƵĐŚƵŶŐĞŶǀŽŶ^ƚŝĞůĐŚĞŶĞƉŝĚĞƌŵŝƐnjĞůůĞŶ

>17(# %+7+ "+ 7 7 "

; 77+ i5 7% + @

6,3 [67 +%

@ 31 3 .777 |y x}HN

2 +>!$%/)'%57 ?

57? %F"7+ 7

ϰ͘ϰWŚLJůŽŐĞŶĞƚŝƐĐŚĞhŶƚĞƌƐƵĐŚƵŶŐĞŶĚĞƌ^ƉćƚďƵƌŐƵŶĚĞƌŬůŽŶĞ

øù úûüýþ ÿû ýû þûüûýû øýûý

þûüûýû üùû þûÿ

þûü$ýû ø ùû ýû %üû &'()* þûúüüûü úûü øýûû #ûýþû! ù ûûûýþûü %üûü ýü! ùû ù #ûýüùüûýüøýûûýþ!ùýû

ϰ͘ϱ/ĚĞŶƚŝĨŝnjŝĞƌƵŶŐǀŽŶ<ĂŶĚŝĚĂƚĞŶŐĞŶĞŶŵŝƚƚĞůƐZE

,$ þûüûýû ,üûýûû ûù#ý

$ùûü ü ûûûýþûü

-)û"üùûü#ýüþûù$ûý(üú úùûü .. +ûüû ýþüýýùüüûýû %üûü &0*!1 ùû ,üûýû! )'û ûýüû û ûûý ûùüüûü 2û#úûýþ 'ûýüûûüþûü$ýûüýý#ýûúûüúù

/ 345 678 9:; <=> ?=@ <=> AAB 9:C 6@= D=E

øùùýü úû þûü$ýû ø ùû û#ýýÿ ûûûýþû%üû!#û -)û"ü þø)û"! úû ûýüûýü#ý Fûúûý ûýüûü ûû#þûü G ûü ûHý$ýûý& IJøýûû+ûü! úû +ûüù$ýýû!ýü ûýû$ûýûü+ûüû ûýüû úý!$ýû+ûüûHûýüûýüûK ù#$ Lûýü û ýûûüýûûü (

ϰ͘ϰWŚLJůŽŐĞŶĞƚŝƐĐŚĞhŶƚĞƌƐƵĐŚƵŶŐĞŶĚĞƌ^ƉćƚďƵƌŐƵŶĚĞƌŬůŽŶĞ

ûþüûüûûùüü ý!úû #ü

øýûû û ù M G! ýû ýû û"ûü *M*! +$)*)N! Oùü Pùý$ù ü Oùü PùýJ ù

ýû $ùûü %üû þûüûý üûýûý ýû ûûûýþûü %üû üûûýüùüû û QRS ST U R UVRRWRVT X

/ĚĞŶƚŝĨŝnjŝĞƌƵŶŐǀŽŶ<ĂŶĚŝĚĂƚĞŶŐĞŶĞŶŵŝƚƚĞůƐZEͲ^ĞƋƵŶĚŐEͲ^ĞƋ

ýüûü! ýû ÿ ûü üýûü ,üûýû #ú þüûüûü ûùüúý ýü! úûü ûþûý

úýüþùýûüù$ YZ[þûÿ&*J\ü ý ûHý$ýû #úýûü ûü û) ü $ùû ] ^!^1J ûýüþ úù üû ýûûü %ùüýùû

þüþûþùûü! úûû # ù þû +û

ùûü

/_`ab=c de f87_ D=Eg_Ch`b=c;;E:a_7= 5E/b8cb=a78_=>`@87;i 7=i7=j a_7 >k8 a_7 9l m 37n 8 g7=a7E gb8a7=o p 9 bq7=a7 ?=@;47 _ r 3 74E7 r

;E78 7`7hE8@=7= r _h8@;h@4: b>=:qr 7 s9Dt p 7_=78 3E `@87;i 7=i:b;8bq7=a7=?=@;47=s8@Ebr 8:=a7EpoBp D_=7 @;47hb8i6@8?=@;4 7=/8bCqofp9Dt b>=:qr 77_=783E `@87;i 7=i:b; 7_= 78 A ABuv ?=@;47s8@E b r 8:=a7EpoDp w 7 Bux v<=>`@8 7;i 7=i hb8i6@ 8 A`kq/7c_==of 78 y >7_`a7bE 7E Cq_;jg 7`Cq 7 :`; 3E:a_brz678 g7=a7E gb8a7o{p 9Dt b = ig 7_ =@Cq c7 ;Cq`@; ;7=7= A`kE7= r _E 3E_7` Cq7 Eg_Ch`b=c;E:a_brAA Buxvo

û ýû þüûüû û"ûü#ýû! $ Kù ûýûûüøûü2ûþûýû%ùüýùûüþûüý

ýþû +ûü ýûüýý#ýû!ù ýü ûýûü ûûûýþûü ü ý$ 2 ûþûý # ûü $ùûü %üûü ýû û ÿ ûýüûü'ù$ûþùýû! þû|# ûüúýýþûü ûþùýûü}ûûýûý#ûü!û Ký-&$ý-Jûüû#ûþýûûü(úûþ

Hûýü û 'ù$ûþù ûûüùûýû

ûýüû #ýûüþ ù$ YZ[ # ûü ýûûü  úýûü ûüû þûù$ ûûýþûü üþûüûü ûüû ý$ 6@= =:`€;7 /78o A p :a_brd 7AABu v :a_br 7_` 7_=7 8 :b> a_ 7 b>=:qr 7 = ib r

$ý-'ù$ûþùþûüûüýû %üû #ûýþûü $ýûû ûû! ûý úû ‚ùüýû |üüûü ùû üý $û û$û! ùýû û|û +ûüûHûý

-ûþùýü$ûùüý$ûùúý

//_`ab=c e t :44_=c48@>_`7 s8@;:p a 78 6 ?:=a_a:E7=c7=7; sc7 `/pot _E u_`>7a783@>E g: a:; :==@E_78E7 ƒ 7_=8 7/7=c7 =@r s„_E_ ;… 6_=_> 78r _EE 7`=o„ 78E_h:`7j>:8/`_Cq7†_=_7=_==78q:`/ E7_`;b=E78 ;Cq_7a`_ Cqig_; Cq7=a7=?`@=7=;_=a

,$ # ûûü! ûýüû û|û (Hû ýûûüÿ! úûù -ûûüþûüù$ üùüþýþûü ‡ýüýûü üüû þû#ûýþ

'ù$ûþùþûü þûü! ýû

%üùü#ûü øû (û úû ü

ˆ‰Š‹ŒŽŒ‘’Š“ŒŠ”Šùü$ýûúû

•–X —U TS˜ SRT

™š Z›TœX RUV

úû ýû $ý- }ýüûûû û 'ù &‚ùûùûüü-ûûûü#ûüJû"ûü

ϰ͘ϲtĞŝƚĞƌĨƺŚƌĞŶĚĞ'ĞŶĞdžƉƌĞƐƐŝŽŶƐĂŶĂůLJƐĞŶ͗

ûü -)û" ûü#ýûüþ û$ úûü .* %ùüýùûüþûüû &%+J

(Hûýüüûýûû ùþûú '

%ûùýü $ý ž\ùüû#! \P22 ‡þü Ÿù#-‚)"GP- ù$  Q[ýü üûü +ûüûHûýüþûÿŸýûÿúûü -û ‚ùûüûüúýüþ ù û$ žù I ûýüûü¡ùüþû¢ü£1ÿI^û¤N ¥Gýüý¦)%üûùüûýü#ûüûüùüû %ùüýùûüþûüû #ûýþûü ù ý$ +

ýû$ý- þûüýûüúûûüùüü øýûûû ûü Fûúûý ûýü G ýü û $ý-)}ýüûûû ýûþ ýû $ý- ûù)ûþýû úûûü ( úý ü ýü ûüûûûýþûü %üûü ýû|üþ

6_ 78 345E/b8cb=a78h`@=7 c7c7= a_7 ?@a_78b=c; ;7nb7= :87B†B§7=@ r _C ; ƒ @8h/7=Cqgb8a7=a_ 79l m3 7n 87:a > 78:o<§ §y… d¨ o6zp h:8E_ 78Ej br a_> >78 7=E_7``7 §7=7 ¨487; /a78y8@>_ `7h 7==i7_Cq=7=q7E 78@i€c@E73l y;ja_7E 7_` ;_a ao

ýü û 'ù$ûþùþûü ùý #

$ YZ[ýü ˆ‰Š‹ŒŽŒ ‘’Š“ŒŠ”Š ÿûûHý$ýû \ü$û úûûü! ù ýü ùüþûüûü Gùü#ûü! ý ýûûü ýþüýýùü üþû úùûü ý$ 2ûþûý # û! ýüû$ ûýüû $ýûû 2ùýùüû û + ûøù$ýùüüùüùþûþùüþûüúûûü!ùýû © TSU ˜ST TY Vª«¬­ª®

WX›T SSVRT¯TR U Tøûú $ûþù ýü *. úûýûûü! ûûûýþûü -û

ü#ýû!úûýFûûýüûGþûüûüúûü

ϰ͘ϲtĞŝƚĞƌĨƺŚƌĞŶĚĞ'ĞŶĞdžƉƌĞƐƐŝŽŶƐĂŶĂůLJƐĞŶ͗

ýûûü .. ýûûüýû ûHý$ýûûü +ûüûü &KG\

ùüùü ýû Oüýü úýû û Ÿ|û

'ûýûû *1 %+ ýü $ý ‚ùûüùýû ù#ýýû ! ùüýûü! üû|ûüýJ øýûû ¤N %+ úûü

ûûüü$ùûü°Gýüý¦%üûüû#ÿþý -ùýûüû%üûüûýùüûü#ù

I^*1 ûúûüû øýû úûüþ û +ûüûHûý %ùüýùûüþûüûúûüü$ùüûýü#ûüûûûü ûü#ûý$$ûü(üúýüþùýûüûþûýû ûüûþûý &û þûþûü $ùJ ûýüûü ÿü

ûûýþûü þ \û

ùû

þ ýûû

#ûýþûü ûýüûü ¡ ùüþû¢ ü £1 ý$ + ùûüùüûüÿGûüùû$žù øýûøùûüùüùùþû#ûýþûÿûýüûýû ùûü QS TUVTT XT ­S– ùüûûýûûýüûü—U TS˜ ™VT

\ü ûýüû$ ‚ùû úûü ¥Gýü +ûüûHûýüû$ýýûûüûýû ýûûüýûû 'ù$üù$ý #úý "GP- ýûü ùüùýû øù (Hûýü #ûýþûûýþýû—U TS˜ ™VTý 2ûþûý$ý$ùûü°Gýüý¦%ü

ϰ͘ϳƌŐĞďŶŝƐƐĞĚĞƌYd>ŶĂůLJƐĞŝŶĚĞƌWŽƉƵůĂƚŝŽŶ'&͘'

//_`ab=cz y@;_E_@=a78²w† ;>k8³=E78 r 78h r c7=7E_ ;Cq7=?:8E7:b ;a78?87 bib=c;4@4b`:E_@ _=/`:ba:8c7;E7``EjA7 787= r 7 8hr :`7_=c8:bb

øýû%üþùûùûGùýü+ }ûûüüþûü$ýûüù$û$ûýûü

#ÿûüKûúûû û‚ù

I^*¤úûü#´‚‡ }ûûüüþûýüþû #úûýžùûýþüýýùüúùûüµ  Z URR›! WT RR› ü ÿ ýû ‡ úûûüøýûûýûþûüùûüP$$ (ýþûüùûüü}ûûûüûûýûü!ú $ýüûûü#úûýžùûù#ýüûü'ûý ýûþûÿ ù ûü P$$ûü .! ¤! ¡Pûüþ¢ û ´‚‡)}ûûýû ù!

+ûüûþûý ûýüû Lûýüû ü ùÿû ýü I^*1

ûûûýûü+ûüûûýüûüŸýüúûýù ûýüGûý!úû – \üûùýü $ý Hýü)(H)Pùýûü ûû ¶RR TUVSS¶RRT RSýüýûý

ý¦ Gûü ù I^*N ÿ ýû 'ýûûü ûü+ûüûüûúûüû(úûúýûû$ûLûýù ûü ûûü ü $ùûü ¥Gýü ý¦)%üûü

$û ûýû +ûüû üüû û#ýû úûûü ûûüýûû‚ùüýûüùü#ýü ùûüûûü% üûüÿûùûùüûýüúûþ

Yd>ŶĂůLJƐĞŝŶĚĞƌWŽƉƵůĂƚŝŽŶ'&͘'ͲϰϳͲϰϮdž͚sŝůůĂƌĚďůĂŶĐ͛

r :`7a78w8:b /7=:8Cq_E7hEb8:b>a7=d·?@44`b=c;c8b447 @=§{o§ m ¸vm ¸ ¨¹„_``:8a/`:=Cºo³=E78r 78h r :`7a789:Cq_ =a†@Ch78/77 8_ch7_E=:Cqa7r»<„f 7 ;h8_4E@8¼¸_=C87r

+O+)¤M)¤IH°2ýùùü¦úûù$ Q[þûü#! ü}üýûü&ûþ‚ùûû*JùûüžùûüI^*1

ûüùýûùûü2üûüþûüü I^

û#\üþûù$#ûýþûüý.N´‚‡!ýûÿû $ýü ¯½  üüûü¾¿À ÿýûGùù$ûû«U

ûûûýþûý üù û$ Á[ à V XT ÄÅÆ û $ûü*!I!M!!**!*¤!*Mü*Oÿ,üûùù$ûû! úùûü´‚‡ùûüP$$ûü1!*^!*I!*Mü*

ûýüúûüÿû#úûýžùû´‚‡ÿ,üû$û$ ! N!~! *^! **! *I! * ü *~ ýûüýý#ýû Ÿýþ $ûûû ´‚‡ ÿ ,üû$û$ùû û‚ùûüù

üù ùü ûûü û øù üþ û $$ý ÿ -‚) žû üûüý$

ϰϮdž͚sŝůůĂƌĚďůĂŶĐ͛

}üýùûüûøûýÇ\2I^¤ù I~Èûüýûü2ùýùü#û øýû $ûùûü Kùû! úûû ýû % þûüûýûü %ùû û$ýû øû ø)û ýû -ûû &G¤^^I¤! žùýü û ù! I^^MJ ýùýûGýýüý$-ûûûü#þûü$

ϰ͘ϴ/ŶƚĞŐƌŝĞƌƚĞĚŝĨĨĞƌĞŶƚŝĞůůĞ'ĞŶĞdžƉƌĞƐƐŝŽŶ

'ûýûýü úûü MI^N +ûüû! ýû -ûûûü#þûü$ û$ýûúûü! $ý û 2û ùþûþýûü~^ +ûüû ûûýüû 2ûùüù#$ý‡ûûûýþûýù ¡ûù Kù¢ øùûüþ û $ýýùý ý¦%üûü#ûýþ!ùû ûüûûü

//_`ab=c¸ Éu7:Er :4Êa7887`:E_67=D¨487; ; h@r 4:hE7=¹y_=@El@_8º?`@=7=ibrAABu3E:a ·¼?:=a_a:E7=c7=7ja_7_=9l m37na_> >78 7=E_7 ²w†mA 787 Cq=b=c`_7c7=o u@8_ i@=E:`:`;§8b44 b=ah@r 4:hE7?`@=7s@/7=pa_7_= a7=Ë:q87= ig_;Cq 7=a7=§8b447=a_7=E:`;<=a_h:E@8>k8;

û$žùI^*1ûþùûýüûü´‚‡ùP$$Iú

%üýûü#ýüûùû û ´‚‡ ùüýûûü! úûü ù û"ûü#ùþûý ýûû Kùû $ýû$-ûûûü#þûü Fý#ýû ýû ‡ùþû û ´‚‡ ÿ ‡ûûûýþûý ù \üüûùû$ùýûûü}ûûýûýûþûüMI^N+

'ĞŶĞdžƉƌĞƐƐŝŽŶ

GFûýü û .N ´‚‡) %üýûü#ýüûùû ù ûü .. ýûûüýûûHý$ýûûü +ûüûüù û$ -ûüù$ýú ý$ ýýüûûüù ù ý$üý #ýýû üúûüù üûû%ùüýùûüþûü ûýüþû ü û%ùüýùûüþûüû ýü ûûüü $ùûü

%ùüýùûüþûüûýü#ýûýüüÿüûý$ýûúû

;_@=s:`;ÌÌ BEƒ 78E7p6@=?:=a_a:E7=c7=7=:b;`@Ch787=b a_brvds 3Cq8 @Eh@8=c8ÍÎ7a78A778 7=po„ 78 E_h:`:b>c7E8:c7 7``7¨48_r _78E;_=ajb=aibc`7_Cq_=a7=?@=>_a7=i_=E786:`` 7 47:b>c7E8:c 7=;_=ar _; Cq/778_c 7sb=E7=pj`@ Ch78/778 _c7s r =¼dxj¼ dÏb =a¼dv/748@/Egb8a7=oD_={:8/c8:a_7=E

;E:/_`7a_>> 787 =E_7``7D¨487 ;;_@=s8 7CqE78A 78 7_Cqp

úûû

ù û

ü$ ÿ ù ýû

ûüû

ù ù

)û" üûûü øýû ü ¥Gýü ûûü

ϱ͘ŝƐŬƵƐƐŝŽŶĚĞƌƌŐĞďŶŝƐƐĞ

2 < 6 @ 5 + 7 77

Y18Z%+ 5/4C+7

1% 27 l;#&( ++ . 7

77C+7 % 6G>47>

++2; %

17 ,+!+%+

2+ 3?+BB9 "373#&&0

27 !7 1+%

+ C74 /=

257C+7

2 >47 + 7 5

/4 1 6G 7

/4 —NVQNKQIMHQJWIN …KPIQNLN“IM“MQRVIN “IM “KMJWPJWNQVVHQJWIN ÚLJWQPHNSI ’IQ HJ—IMIN KN“ —”TL—VIN |RHLN•IN , "373 #&&0 +% 77

Y. Z Y?4ZD C+; 7+ C+Y"Z

Y!=Z+3@ +

2 > + Y1 8Z

++2C74/=+

#&9#&' > 777Y1

8Z + " >+ + 7

!=/677\]^_`abcd`d/

/ !=3 .8, 5 1 #&' !

7 % 7 !=

> .8, >+ + 3

>+ .8, 7 2 6 !=

7+ 7 "81 7 % +

% .8, +% / 2

.> 7.8,"

<+;<51 #&' !

++ 7 3 .8, > 76 6

7+ Y1 8Z C 7

.777 %7+ 76

, ! 3 , 4 !+

1% 57% !7 7 C+7

3 2 6 ,73 >

143%7<\]^_`abcd`def^g`^h^ %

k+=> "

C+7 7= <7% 7 3

121+ ,7 "

76 3 " "

F 57% . % 1 6G%

"7 3 %

4 ,4 2 23 7%

"81 F + <

% .8,+6 2

. 6%!+5 "

! < > 76 <

% .+ @7+ 7 2 "X

3 0 7+ .+ 7

< " +7% 1

7+ 14 3 > 76 /

> / + + . 3

7C+73

2 F ; C+7 + Y1 8Z 7

% <7 . % 1 !+3

G + 4 i 7 77 Y1 8Z

"% + C+ 3 %

+ 4 i 77+ +

7+ C+ @ ."373

#&&0 5 % 7 !

.3 6 , G +Y1

8Z1% 1F7 2 % ,+ 7

/4l 3<4i

3 76 2 +% i 3 7 <

++7C+76

2՘օNLHOPI3 953+

3 1 4

#&' C75#&( 3214

1 ; <7C+72 C%

0' *C@ C+77 $

76%=<72@l2>

#%'( + @ 8++ %&$ @7+ 7

l;#&( 22@l2> +(%'9 *C@ i7

C+7 C+7 ? #&( 3 + @l2

> @l2> 3 9%# , , B

*C@ #0 57 3 + + 7=

2@7+ 7

ϲ͘ ŶŐĂďĞŶ njƵŵ ǀŽƌĂƵƐƐŝĐŚƚůŝĐŚĞŶ EƵƚnjĞŶ ƵŶĚ njƵƌ sĞƌǁĞƌƚďĂƌŬĞŝƚ ĚĞƌ

ƌŐĞďŶŝƐƐĞ

2i 1F7+ %3 57%3

?>+ %@7+ 7+!

7=<7!78 <7

% ! 3 ? !7 3

% + , k+ +7

. G+ 1

*C@ ,4 i7 C+7

F o? p "+<7 3

. 3 ,++ 0 2 F 7 7 <7

3 + 3

, 7 7% 3 % ;

57 + 2 , : 3

;3 ! % . 1+7

57 2 7<7

k+ +7 @7+ 7 G+ =

12,+1F7

7 1 ; 2+ 7

.+ / i

2 /=4 "+ 7 + @7+ 7

= /4

% 7 !7 5

+ 73 2 / 5

1 7[77 14 C+7 ,73

23214 3.8,

+ > + "+ 6

k+ 14<7@7+ 7

7 .+ , + 0 X ;

7+ /4 !3 / .+

4 >7 7 "

1F7 4 /

7= <7 @7+ 7 + 2 4

?7 17 %57. %

1 6GC+7+ /2

7 1 *C@ ,4 7

"+ 7 = 7 /4 C+7

>

!7 , <7 @7+ 7

"+"+ 777< 7%

ϳ͘ 'ĞŐĞŶƺďĞƌƐƚĞůůƵŶŐ ĚĞƌ ƵƌƐƉƌƺŶŐůŝĐŚ ŐĞƉůĂŶƚĞŶ njƵ ĚĞŶ ƚĂƚƐćĐŚůŝĐŚ

ĞƌƌĞŝĐŚƚĞŶŝĞůĞŶ͕,ŝŶǁĞŝƐĞĂƵĨǁĞŝƚĞƌĨƺŚƌĞŶĚĞ&ƌĂŐĞƐƚĞůůƵŶŐĞŶ

óK…| 14?7

-2 C+7 ; 3 i7 3 C+

"+ 7% . l; % = 14 Y?Z

5 1++

óK …|Ž ?7 3 / +7

; <

.+-l /+7; < 7

.+2 .8,"X,47

!= 2 i 23 28, 3

7[77"+ 7 C74

@7+ 7>+

óK …|Œ 2 /=4 7 3

@7+

7-2 /= .8,"X ,4

"+ 7 i !=

"+ "+ 7+ %

=/4 %

, 3 , >

3 %+ +5 C77 % 1

>

óK …|œ *C@ ,4 3 <7 @7+ 7 1 //,($(# = Ð;

+Ñ-2 *C@ ,4 3 i7 @7+ 7 0' +

*C@ +%??>+33

,++ 23%: ; ;3*C@

. % + 7<7

.+ +

óK…|; 3 @7+ 7+

/=-, /=4 +.8,"X2%

+ @7 *C@" + /

% @7+ 7 +

óK…|›/33 <77

-/3 % + % + "+ 7

"X 2 i + 7+

+ .8,% 57 3

"X 6" / C77

3 73%3>1

57 2 . %

C+ 7 2 , < 7+

< :7++

ϴ͘ƵƐĂŵŵĞŶĨĂƐƐƵŶŐ

1F7 4 /

7= <7 @7+ 7 + 2 4

?7 7 % 57 . % 1

6G C+7+ / 2 7

1 *C@ ,4 7

"+ 7 =7/4C+7

>

!7 , <7 @7+ 7 " +

"+ 77:7< %

@7+ 7 7 + < "

C+7

ϵ͘>ŝƚĞƌĂƚƵƌǀĞƌnjĞŝĐŚŶŝƐ

?%! C%<%@,%,3?BB9 23

mbe]ne`d+ +14 4)9

-B$&#-&&B([14)9B$

?+8%5<%@ @%.;#&9 5 "%C%

>2/3Ò 4?+4

mbe]ne`dq`hr]r^14 4&90 -0#9000-&&B([4&9(&9#

? "%,5BB) 5-\Ó]b_^qer]`tÔ

\]^_`abcd`def^g`^h^1'' -$09$(0-&&('[F0'900=BB)&&0(0=

?1% @%/%2BBB <

mbe]ne`dq`hr]r^+4 +414 <1

1 499 -0$(0--[[ [&&&'[BBB&#&'

?+/BB9 /" /3-,44 3

,/>.# -&(& -&[F$99&#0)BB9+&&&)'=

?%<<%<!?%@+2%<?%1<%:1#&(

:+4*C@4

3`e`d€`h`sr]^@ C,/C

C /7#$9 -(0'#-&&&$[&&##&(##)'4 271%5%<"%,?%:4%@+%@ @BBB 2

3+4mbe]ne`dq`hr]r^14 4)B

0 -B$#&0-&&B([4BBB)B0B$

5%:@%4!%2<%: 2%>%C.#&( *C@4

7

`e`dC,/#$B -)9$)$#-2l&&&$[&&##&( #0&#

5@%C @%" ;%X,%<<#&& C

3 3 3C

1F+ 4'( -9(999$-&[F0'9

00Õ#&&B&(&B&=

51%1 ."#&' C.8,3 33?

l1 4#B-'($#

l%,4<%8%1,%??%? ,%?8%,+ "%;8%+?% ;,%@ 5%: 41%23?%:2%<!%+2%1%4?% ,%" %/43=<%i !%?5%,;%;;%2 5++?%,=<%2//%2;%58%1"%%<<%

"+"%? ,%@?%</%2!%1/%@;%?1% < 2%2<%1<%@4,%"?%, 35%1<!%; /%< <%?+<%,,5%>+%*5%>71%1+

5#&&$ C 3 X =F

48((B-('0i('9

,%..%: %Ö"%56>%C6.#&0 ,C4,4C -,

X+%%3

3+;9#0 -#B09

<%:<#&&) 5 34mbe]ne`dq`hr]r^

34/412199 -#(#B

<%4::%:<#&&$ , 4+4

3 3+47+4mbe]ne`dq`hr]r^

;!? "41C 1@"l8!)# -)0&(0 -[[ [&0$[F&&)0&(0

"?,%.+>"%!>#&# 8: -#94 4 8<B$ -'$'$9-&&0)[#&)B

"373Ò8%24%C<.#&&( +3`e`d €`h`sr]^@,/>.&# -'#(

-&[F$99&#0)#&&(+&&&(=

C31"#&&B .7+4mbe]ne`dq`hr]r^1-5

?121'# -$0$$

;<!%<BB /+4+mbe]ne`d

q`hr]r^14 4)# -))B-&&B([14)))

;lF>%<*C@'%" X3=

4;~> %8-#&&B

>%C47%C471%3,@#&&$ mbe]ne`dq`hr]r^- 4 <11 4)9 -9'9)&-&[F0'(0$&0#&&$&&($= 4!%l%"5%""%:@%.=<%<"1%/ <"%>< "%< 2%!+.%C6.#&' *3

3</

/#B( -9$09B(-&&&$[&&(0)&'#&&9

ϭϬ͘mďĞƌƐŝĐŚƚnjƵsĞƌƂĨĨĞŶƚůŝĐŚƵŶŐĞŶ͕ƌĞĂůŝƐŝĞƌƚƵŶĚŐĞƉůĂŶƚ

L†ÚIRIMQIMVI|K’HQ—LVQNINQN×LJW•IQVPJWMQRVIN

/-.%.%C6%C%

4-2+4++*C@<,"

.%.%"%"+ %C6%4%

C-, 3

.%.%C6%C%

4-2 = 3+

’†ÛQJWV×MIRIMQIMVI|K’HQ—LVQNIN

?1+&(4#&$

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

/4 3

l>+?B%&&'#&$

J†|PVIM

1B#&'

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

14 4 3

/.5'3j18Z

Ò #&'%*+

1#0#'&##&'

.%.%C6%4%C

C 4

3

#BC <7+ 1%2+

“†ÚMVMSI

; #B&)#&$

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

<7,4C+7<,C,

C ,/Bl+%/6%.+/1 /1 %"+

; &&'#&$

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

/,4@?,/3

.+. Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

<74i @7+ 7+.+

52>C #&$%/

; 0&&#&$

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

?3+ 33j18Z

1 "i3/G%/G

; (&(#&'

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

i @7+ 7.+%5747mbe]ne`d5 7

52>C #&'%

; #0&##&'

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

144 3

#B+ 4%2+

; #&&#&9

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

,4+ 3

85#&9%*+

; (&#&9

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

,4+ 3

1 " i3/G%/G

; #)&(#&9

.+.

Ø

%".

Ù

%.C6

Ø

%C

Ù

!34

Ø

<,C,<7,4C+7

52>C #&9%l

Ø

.+ /%"+

Ù