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The Short Stalk Length of Highly Pathogenic Avian Influenza H5N1 Virus Neuraminidase Limits Transmission of Pandemic H1N1 Virus in Ferrets

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Figure

FIG 1 Sequence alignment of human, avian, and engineered NAs. Sequences of N1 NA are shown by full-length amino acid sequence (A), nucleotide sequenceof region covering stalk deletion (B), and amino acid sequence of stalk region (C)
FIG 2 Multicycle virus replication of SStyNA is attenuated in cell culture.
TABLE 1 Mean difference between amounts of virus shed, day virus was first shed, and number of days that virus was shed from ferrets afterexposure to LStyNA in comparison to SStyNAa
FIG 4 Viruses with short-stalk NA are eluted slowly from red blood cells.Concentrated viruses were diluted to equal HAU and then mixed with RBCs at4°C to promote hemagglutination
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