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Visualization of Intracellular Movement of Vaccinia Virus Virions Containing a Green Fluorescent Protein-B5R Membrane Protein Chimera

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Figure

FIG. 1. Plaque phenotype of vB5R-GFP. Viruses were plated on monolayers of BS-C-1 cells
FIG. 2. Synthesis of B5R-GFP. HeLa cells were infected with WRor vB5R-GFP. At various time points, cells were harvested and ana-
FIG. 4. Immunogold electron microscopy of cells infected with vB5R-GFP. RKfixed in paraformaldehyde, cryosectioned, and incubated with GFP polyclonal antibody followed by 10-nm-diameter gold particles conjugated to13 cells that had been infected with vB5R-G
TABLE 1. Frame-by-frame measurements for five individual virions
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