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Increased Antibody Affinity Confers Broad In Vitro Protection against Escape Mutants of Severe Acute Respiratory Syndrome Coronavirus

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Figure

FIG 1 Comparison of fluorescence histograms. E. coli spheroplasts were labeled with 20 nM RBD, and binding was detected with 200 nM anti-FLAG PE
FIG 2 Sequence comparison of the engineered, high-affinity variants RS2, SK4, and RSK with parental 80R scFv
TABLE 3 Amino acid differences in the RBD of the spike protein
FIG 5 SK4 neutralization of escape variants. Neutralization activity of SK4 was tested against escape variants, and the IC50s were compared to that of icUrbani.
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