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A Peptide Derived from RNA Recognition Motif 2 of Human La Protein Binds to Hepatitis C Virus Internal Ribosome Entry Site, Prevents Ribosomal Assembly, and Inhibits Internal Initiation of Translation

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Figure

FIG. 1. Effect of deletions on the binding of La-RRM2 to HCV IRES RNA. (A) Schematic representation of N- and C-terminal deletionswithin La-RRM2
FIG. 2. Ability of the peptide LaR2C to bind to HCV IRES RNA. (A) The sequences of the peptides LaR2C, NSP, and NSP-La are indicated.The LaR2C, NSP, and NSP-La peptides were analyzed by resolving by SDS–12% Tris-Tricine gel electrophoresis followed by silver staining.
FIG. 6. Effect of TAT-LaR2C fusion protein on HCV IRES-medi-ated translation in vivo. (A) Schematic representation of the TAT-
FIG. 7. Effect of LaR2C peptide on ribosomal assembly on the HCV IRES RNA. Sucrose gradient sedimentation profiles of indicated

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