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Conformational Changes in the Adenovirus Hexon Subunit Responsible for Regulating Cytoplasmic Dynein Recruitment

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Figure

FIG 1 Low-pH priming of hexon for dynein binding is reversible. (A) Immu-noisolated hexon was acidified, returned to neutral buffer, and kept at neutral-ity for the indicated periods of time on ice before it was incubated with purifieddynein for 1.5 h at 4°C
FIG 2 HexondissociationeffectsoflowpHandSDS.(A)PurifiedAdV5hexontrimersweretestedforlow-pHdissociation.FollowingexposuretopH7.4or4.4,hexonsampleswereincubatedinproteinsamplebufferovernight,subjectedtoSDS-PAGEwithoutboiling,andstainedbyusingCoomassieblue.Dis
FIG 3 Kinetics of SDS-mediated hexon dissociation and reassociation. (A) To test the kinetics of SDS sensitivity, purified hexon was incubated at pH 4.4 for 30min and then exposed to PSB for the indicated time periods
FIG 4 Dispase hydrolysis inhibits low-pH effects on hexon structure and dynein binding
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