The C Terminus of Rotavirus VP4 Protein Contains an Actin Binding Domain Which Requires Cooperation with the Coiled-Coil Domain for Actin Remodeling

Wilfried Condemine,a_{Thibaut Eguether,}a_{Nathalie Couroussé,}a_{Catherine Etchebest,}b_{Agnes Gardet,}a_{*Germain Trugnan,}a Serge Chwetzoffa,c

a_{Sorbonne Universités, UPMC University Paris 06, École Normale Supérieure, PSL Research University, CNRS, INSERM U1157, APHP, Laboratoire des Biomolécules (LBM),}

Paris, France

b_{Equipe DSIMB, Dynamique des Structures et des Interactions des Macromolécules Biologiques, INTS, INSERM-Paris-Diderot UMR-S 1134, Paris, France} c_{VIM INRA Domaine de Vilvert, Jouy-en-Josas, France}

ABSTRACT The interactions between viruses and actin cytoskeleton have been widely studied. We showed that rotaviruses remodel microfilaments in intestinal cells and demonstrated that this was due to the VP4 spike protein. Microfilaments mainly occur in the apical domain of infected polarized enterocytes and favor the polarized apical exit of viral progeny. The present work aims at the identification of molecular determinants of actin-VP4 interactions. We used various deletion mutants of VP4 that were transfected into Cos-7 cells and analyzed interactions by immunofluores-cence confocal microscopy. It has been established that the C-terminal part of VP4 is embedded within viral particles when rotavirus assembles. The use of specific mono-clonal antibodies demonstrated that VP4 is expressed in different forms in infected cells: classically as spike on the outer layer of virus particles, but also as free soluble protein in the cytosol. The C terminus of free VP4 was identified as interacting with actin microfilaments. The VP4 actin binding domain is unable to promote microfila-ment remodeling by itself; the coiled-coil domain is also required in this process. This actin-binding domain was shown to dominate a previously identified peroxi-somal targeting signal, located in the three last amino acids of VP4. The newly iden-tified actin-binding domain is highly conserved in rotavirus strains from species A, B, and C, suggesting that actin binding and remodeling is a general strategy for rotavi-rus exit. This provides a novel mechanism of protein-protein interactions, not involv-ing cell signalinvolv-ing pathways, to facilitate rotavirus exit.

IMPORTANCE Rotaviruses are causal agents of acute infantile viral diarrhea. In intes-tinal cells,in vitro as well asin vivo, virus assembly and exit do not imply cell lysis but rely on an active process in which the cytoskeleton plays a major role. We de-scribe here a novel molecular mechanism by which the rotavirus spike protein VP4 drives actin remodeling. This relies on the fact that VP4 occurs in different forms. Be-sides its structural function within the virion, a large proportion of VP4 is expressed as free protein. Here, we show that free VP4 possesses a functional actin-binding do-main. This domain, in coordination with a coiled-coil domain, promotes actin cyto-skeleton remodeling, thereby providing the capacity to destabilize the cell mem-brane and allow efficient rotavirus exit.

KEYWORDS VP4 protein, actin binding, actin remodeling, protein domain, rotavirus

R

CitationCondemine W, Eguether T, Couroussé N, Etchebest C, Gardet A, Trugnan G, Chwetzoff S. 2019. The C terminus of rotavirus VP4 protein contains an actin binding domain which requires cooperation with the coiled-coil domain for actin remodeling. J Virol 93:e01598-18.https://doi.org/10.1128/JVI.01598-18.

EditorSusana López, Instituto de Biotecnologia/UNAM

Copyright© 2018 American Society for Microbiology.All Rights Reserved.

Address correspondence to Germain Trugnan, germain.trugnan@upmc.fr, or Serge Chwetzoff, serge.chwetzoff@upmc.fr.

*Present address: Agnes Gardet, Biogen, Massachusetts, USA.

G.T. and S.C. contributed equally to this work.

Received11 September 2018

Accepted10 October 2018

Accepted manuscript posted online17 October 2018

Published10 December 2018

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external being composed of assemblies of the VP7 coat and VP4 spike proteins (3–5). Rotaviruses infect enterocytes from the luminal side. After replication, new virions are released at the apical pole by an atypical polarized trafficking pathway that has been partially described (6). The VP4 spike protein is reported to play a major role in both rotavirus entry and exit thanks to several molecular interactions. VP4 is indeed thought to mediate primary interactions with cellular components through its N-terminal subunit, VP8*, which can interact with cell surface receptors (7, 8) and/or coreceptors, which contain specific glycan moieties (9). VP5*, the C-terminal subunit of VP4, has been shown to interact with several putative partners, including integrins (10), mem-branes via a fusion domain which allows virus entry (11), other VP4 molecules to form oligomers (12), and peroxisomes (13). Previous studies have shown that VP4 also specifically interacts with the apical actin cytoskeleton and induces its remodeling, leading to a disorganization of the brush border (14). We showed that VP4 interacts with actin microfilaments by perturbing their treadmilling-based renewal, a specific mechanism involved in actin dynamics (15), and we have proposed that this favors the access of virions to the apical pole of enterocytes for cellular exit (14), since the actin network is essential for exocytosis (16). Finally, we demonstrated that blocking the treadmilling by using jasplakinolide leads to a fully depolarized release of new virions (15). A similar process has been recently demonstrated by another group in MA104 cells (17), a nonpolarized cell line, indicating that our initial observation is not restricted to polarized enterocytes.

Actin microfilaments represent a common tool in the wide spectrum of strategies developed by viruses to take control of their target cells (18). Some viruses, such as vaccinia virus, baculoviruses, Epstein-Barr virus, herpes simplex virus, frog virus 3, and murine mammary tumor virus, promote the polymerization of these cytoskeletal fila-ments to assist their intracellular movefila-ments, assembly, and budding (19). In some instances, F-actin projections are induced to promote moving of viral particles and budding, as seen with measles virus, vaccinia virus, or p815 retrovirus (20). Human immunodeficiency virus type 1 (HIV-1) induces cell shape changes and the generation of intercellular synapses, involving actin microfilaments, to facilitate virus budding and spreading (21). Baculoviruses also induce the localization of actin toward an ectopic compartment, the nucleus (22). Other viruses, such as influenza virus, only show colocalization of some of their proteins with actin cytoskeleton (23). Actin or actin-related proteins have also been considered a structural component of the viral particle for rabies virus and HIV-1 (24, 25). At least some viruses use actin as cofactors for their replication, e.g., human parainfluenza 3, where polymerized actin interaction with the transcribing ribonucleoprotein is required for mRNA synthesis (26). Interestingly, only a few viruses, such as adenovirus, human cytomegalovirus, vesicular stomatitis virus, and rubella virus, have been shown to disrupt actin microfilaments, by inducing their cleavage, to facilitate virion release (27–30).

Our previous work underlined the crucial role of the spike rotavirus protein inter-action with actin (14, 15). However, mechanisms of actin binding and remodeling by VP4 remained unknown. Analysis of the primary amino acid sequence of VP4 did not reveal any already identified actin-binding domain or any homology with known actin-binding protein. In the present work, a detailed molecular dissection of the VP4 sequence was performed in order to define how VP4 interacts with actin microfilaments and how it promotes remodeling. A new actin-binding domain was discovered in the C-terminal part of VP4, which we named rotavirus actin-binding domain (RV-ABD). When expressed alone, this motif does not induce obvious reorganization of the actin cytoskeleton. Microfilament remodeling requires the cooperation of RV-ABD and the coiled-coil domain of VP4. Interestingly, these domains are buried within the viral particles (5), but we were able to shown here that a pool of free, nonassembled VP4 is responsible for its actin binding and remodeling activities. Bioinformatics studies indicate that this RV-ABD was present in several strains of species A, B, and C rotavi-ruses, suggesting that actin binding and remodeling is a general strategy for rotavirus replication. Our results describe a novel and original pathogen-driven microfilament

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reorganization strategy based on simple protein-protein interactions, without the involvement of signaling pathways to facilitate virus exit from the cells.

RESULTS AND DISCUSSION

VP4, the rotavirus spike protein, is expressed both within virions and as a free protein.Previous observations have shown that the spike rotavirus protein VP4 is able to bind and remodel actin during rotavirus infection of MA104 and Caco-2 cells (14, 15). To understand the molecular mechanisms of this remodeling process, we first analyzed the subcellular localizations of VP4 during infection by using two distinct monoclonal antibodies (MAb): 7.7, a neutralizing antibody described to mainly recognize VP5*in both free and assembled VP4 (31, 32), and 2G4, an antibody that recognizes VP8*when assembled within the viral particles (33). Confocal microscopy and double immunola-beling of RV-infected MA104 cells showed that these two antibodies did not display identical patterns (Fig. 1). A large part of VP4, as revealed by 7.7 MAb, was distributed along filament-like structures, reminiscent of actin microfilaments, as previously ob-served (14), as well as in dots that did not colocalize with 2G4 staining. VP4 was also present in dots that were labeled by 2G4 MAb but only partially by 7.7 MAb. This pattern corresponds to the final steps of rotavirus assembly in these cells (15). The results indicate that VP4 is always expressed in three forms: (i) assembled with virion progeny, (ii) along intact actin microfilaments, and (iii) within remodeled microfila-ments. These results also confirmed that 2G4 MAb only recognizes VP4 when assem-bled in rotavirus progeny particles and that rotavirus-infected cells always express VP4 both as assembled virions and as a free protein.

The C-terminal part of VP4 contains signals for actin binding and remodeling. Since VP4 is involved in actin reorganization and most of the VP5* subunit is buried within the viral shell in assembled rotavirus (5), we hypothesized that the effects of VP4 on actin microfilament reorganization are either due to the VP8*-containing domains on the outer and middle layers of virions or to VP5* from the nonassembled VP4. However, results from Fig. 1 indicated that the antibody recognizing VP8*, 2G4, never stained filament-like structures, indicating that VP8*is unlikely to be involved in actin reorganization. Therefore, we decided to focus subsequent experiments on the VP5* subunit of VP4. To delineate the molecular bases of actin binding and reorganization, we designed a strategy in which we expressed VP4 or fragments thereof outside the context of infection. To this end, Cos-7 cells were transiently transfected by a series of VP4 constructs (Fig. 2) that will be detailed below. Control experiments (Fig. 3) demonstrated that the addition of green fluorescent protein (GFP) does not modify VP4 FIG 1VP4 is expressed both within virions and as a free protein. MA104 cells were infected by rotavirus RF stain and analyzed by confocal microscopy and immunofluorescence at 6 h postinfection (h.p.i.) using two different monoclonal antibodies. MAb 7.7 (A and D) mainly recognizes VP8*either on assembled or unassembled VP4, and 2G4 (B and E) recognizes assembled VP5*. Panels C and F represent merged images of panels A and B and panels D and E, respectively. MAb 7.7 staining was revealed using FITC-labeled secondary antibody (green), and 2G4 staining was revealed using Alexa-547 dye (red) directly coupled to the antibody. Arrows point to some 2G4-labeled dots that colocalize faintly with 7.7 staining. Arrowheads point to some 7.7-labeled dots that do not colocalize with 2G4 staining. Scale bar, 5␮m. Images are representative of three independent experiments.

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expression and localization, as shown by colocalization between VP4-GFP and MAb 7.7 staining. Figure 3 also shows that 2G4 is unable to recognize VP4-GFP, confirming that 2G4 only recognizes the virion-assembled form of VP4. As expected from previous results, VP4-GFP displayed both a filament-like and a punctate structure. VP8*-GFP is expressed in the soluble fraction of the cell, again making its role in actin remodeling highly unlikely. Interestingly, VP8*-GFP was also recognized by 7.7, suggesting that this antibody can also interact with a portion of VP8*.

Various VP4 deletion constructs were produced (Fig. 2) and then tested by trans-fection in Cos-7 cells, followed by confocal microscopy analysis. The first three con-structs consisted of the removing of either the 480 amino acids (aa) of the N-terminal part, keeping the coiled-coil domain, the first 561 aa, removing the coiled-coil domain (aa 481 to 574), or keeping only the C-terminal part (aa 718 to 776) of VP4. These three constructs provided patterns very distinct (Fig. 4) from those of the full-length VP4 FIG 2Summary of the VP4-GFP constructs used in the present study. Each construct was obtained using the backbone plasmid pEGFP-C1, and all truncated-VP4 constructs encoding amino acid stretches 481 to 776, 481 to 773, 481 to 757, 562 to 776, 641 to 776, 641 to 773, 641 to 757, 702 to 776, 713 to 776, 713 to 773, and 718 to 776, as well as the constructs containing deletions (481-776 del 574-670, 481-776 del 574-713, and 481-776 del 574-718), were generated by PCR and inserted within pEGFP-C1 vector via EcoRI and BamHI sites. Sp100 coiled-coil fused to VP4 (562-776) was made by insertion of PCR-amplified Sp100 coiled-coil 29-152 in VP4 (562-776). Note that GFP-truncated VP4 encoding amino acid stretches 713 to 776, 718 to 776, 718 to 773, and 733 to 776 were made by insertion of amplified segments from the SA11 strain in pEGFP-C1 via XhoI and BamHI sites.

FIG 32G4 antibody only recognizes assembled VP4. Cos-7 cells were transiently transfected using either a VP4-GFP construct or a VP8*-GFP construct. Three days later, cells were fixed, permeabilized, and analyzed by confocal microscopy and immunofluorescence using 7.7 or 2G4 MAbs. Transfected GFP constructs appear in green, and Alexa-547-labeled secondary antibodies (red) were used to reveal MAb 7.7 or 2G4. Scale bars, 5␮m. Images are representative of two independent experiments.

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construct, which displayed both filament-like structures and puncta that colocalized with phalloidin staining (Fig. 4A to C). Transfection of the 481-776 construct mainly displayed large puncta and a few filament-like structures that also colocalized with phalloidin (Fig. 4D to F). Removal of the first 561 amino acids resulted in a pattern where the VP4 fragment colocalized with nonremodeled actin microfilaments (Fig. 4G to I). Finally, the C-terminal VP4 construct displayed a distinct pattern with several small dots that did not colocalize with actin microfilaments (Fig. 4J to L). These results suggested that (i) a domain in the C-terminal part of VP4 is required to promote interactions with actin microfilaments; (ii) binding to actin microfilaments and micro-filament remodeling should be independent, since it was possible to observe actin-VP4 colocalization without any puncta (Fig. 4G to I); and (iii) the most C-terminal part of VP4 contained another signal promoting interactions with another subcellular compart-ment that will be characterized in the following paragraph.

A novel mechanism for virus-microfilament interactions.The above-described results indicated that (i) the full-length form of free VP4 both promotes colocalization with actin microfilaments and induces microfilaments remodeling, (ii) the N-terminal part of VP4 is not involved in interactions with actin microfilaments, and (iii) the C-terminal part of VP4 contains a signal(s) necessary for colocalization with microfila-ments (Fig. 4G to I). The next step was to define more precisely the VP4 domain involved in actin binding and to explain the molecular mechanism of microfilament remodeling. Several other constructs, also diagrammatically shown in Fig. 2, were tested using transient transfection of Cos-7 cells to delineate a minimal putative rotavirus-actin-binding domain. As shown in Table 1, colocalization of VP4 constructs with actin microfilaments was always observed, except for 2 constructs, namely, FIG 4Effects of successive N-terminal deletions on the colocalization of VP4-GFP constructs with actin microfilaments. Cos-7 cells were transiently transfected using four RF VP4-GFP constructs (green) (A, D, G, and J) containing either the full-length VP4 (construct 1-776) (A to C), an N-terminal deletion keeping the coiled-coil domain (construct 481-776) (D to F), an N-terminal deletion removing the main part of the coiled-coil domain (construct 562-776) (G to I), or a construct containing only the last 59 C-terminal amino acids of VP4 (construct 718-776) (J to L). Three days after transfection, cells were fixed, perme-abilized, labeled with Alexa-547-phalloidin (red) (B, E, H, and K), and analyzed by confocal fluorescence microscopy. Panels C, F, I, and L represent the corresponding merged images. Scale bar, 5␮m. Images are representative of four independent experiments.

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713-758 and 718-776, indicating that RV-ABD should be located between aa 713 (Thr) and aa 773 (Gln) of the RV strain. Interestingly, Table 1 also indicated that microfilament remodeling was only observed when the expressed fragment contained the entire coiled-coil domain together with RV-ABD. The coiled-coil domain by itself was unable to promote microfilament colocalization and consequently their remodeling. Transfec-tion of VP4 constructs containing the coiled-coil domain and RV-ABD (aa 481 to 776) (Fig. 4A to C and 5A to C) resulted in actin remodeling, whereas transfection of VP4 constructs in which the coiled-coil domain has been removed while the RV-ABD has been conserved resulted in actin colocalization without remodeling (Fig. 4G to I and 5D to F). Transfection of VP4 constructs with internal deletions allowed us to better define the precise limits of RV-ABD. As shown in Table 1 and Fig. 5, the VP4 constructs 481-776 del 574-670 (Fig. 5G to I) and 481-776 del 574-713 (Fig. 5J to L), in which both the coiled-coil and the RV-ABD domains are conserved, were still able to remodel actin microfilaments. In contrast, removal of both the coiled-coil and the RV-ABD domains (construct 481-776 del 574-718) suppressed both microfilament colocalization and actin remodeling (Table 1). Together, these results strongly suggested that VP4 is able to bind actin microfilaments via its RV-ABD (aa 713 to 773) and to remodel actin microfilaments through the joined interaction of both the coiled-coil domain and the RV-ABD. To demonstrate this, we used two distinct approaches. First, a construct containing the rotavirus RV-ABD fused to an irrelevant coiled-coil domain from the nuclear antigen Sp100, a component of PML nuclear bodies (34), was transfected in Cos-7 cells and resulted in both microfilament colocalization and actin remodeling, as shown on Table 1 and Fig. 5M to O. Second, Cos-7 cells were transfected with two VP4 481-776 constructs inserted either in GFP- or mCherry-containing plasmids. As ex-pected, these two constructs displayed perfect colocalization (Fig. 6A to C). This model also allowed us to perform fluorescent resonance energy transfer (FRET) experiments in which cells were illuminated only with a blue light (488 nm) to excite GFP, and fluorescence was collected either in the green range (500 to 530 nm) or in the red range (570 to 650 nm). As shown in Fig. 6D and E, in cells where both VP4-GFP and VP4-mCherry constructs were expressed (Fig. 6D, upper row), a large proportion of the fluorescence was recovered in the red range, indicating that the two constructs were in close interactions, allowing energy transfer (FRET) from GFP to mCherry. This strongly suggested that they oligomerize even when expressed outside the context of viral infection. In contrast, in control cells, where GFP- and mCherry-containing constructs were transfected without VP4 (Fig. 6D, lower line), a small proportion of FRET could be TABLE 1Actin microfilament binding, remodeling capacities, and peroxisomal targeting of different VP4 constructs used in this studya

Construct

Microfilament remodeling

Microfilament binding

Peroxisomal localization

1-776 ⫹ ⫹ ⫺

481-776 ⫹ ⫹ ⫺

562-776 ⫺ ⫹ ⫺

574-776 ⫺ ⫹ ND

670-776 ⫺ ⫹ ND

702-776 ⫺ ⫹ ND

702-773 ⫺ ⫹ ⫺

713-776 ⫺ ⫹ ⫺

713-773 ⫺ ⫹ ⫺

713-758 ⫺ ⫺ ⫺

718-776 ⫺ ⫺ ⫹

773-776 ⫺ ⫺ ⫹

481-776 del 574-670 ⫹ ⫹ ND

481-776 del 574-713 ⫹ ⫹ ND

481-776 del 574-718 ⫺ ⫺ ND

Sp100 CC-VP4 574-776 ⫹ ⫹ ND

a_{Microfilament binding and remodeling capacities were evaluated by confocal microscopy. ND, not}

determined.

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detected. Quantification of fluorescence in each channel (Fig. 6E) confirmed these results.

The present results indicate that actin remodeling results from the concerted action of two distinct domains, i.e., RV-ABD and coiled-coil. The most likely molecular mech-anism by which VP4 remodels actin microfilaments is that at least two molecules of VP4 bind to actin microfilaments through RV-ABD domains and oligomerize through coiled-coil domains, allowing microfilament bridging. The simultaneous occurrence of the RV-ABD and coiled-coil domains within a single protein chain should promote VP4 oligomerization and in the meantime will induce the remodeling of preassembled F-actin bundles. Such an interaction results in the formation of what we previously called actin bodies (14), which are focal clusters of F-actin fully colocalized with phalloidin. This mechanism explains how VP4 may induce a destabilization of the subcortical microfilaments, favoring the crossing of viral particles through the apical cell membrane and their release in the extracellular space without significant cell death (6, 17). This mode of action of VP4 is in agreement with the modifications of the actin cytoskeleton previously observed during rotavirus infection of MA104, Cos-7, and Caco-2 cells, where microfilaments are progressively gathered in actin bodies (14, 15). These cytoskeleton modifications observed during rotavirus infection are also observed during cellular transfection of the pEGFP-C1-VP4 expression plasmid (14), meaning that FIG 5Role of RV-ABD and coiled-coil domain in actin binding and remodeling. Cos-7 cells were transiently transfected using five of the RF VP4-GFP constructs described in the legend to Fig. 3, namely, constructs 481-776 (A to C), 670-776 (D to F), 481-776 del-574-670 (G to I), 481-776 del-574-713 (J to L), and the construct containing the exogenous coiled-coil domain Sp100 CC VP4 574-776 (M to O). Three days after transfection, cells were fixed, permeabilized, labeled with Alexa-547-phalloidin, and analyzed by confocal fluorescence microscopy. Panels A, D, G, J, and M represent GFP fluorescence (green); panels B, E, H, K, and N represent phalloidin fluorescence (red); panels C, F, I, L, and O correspond to the respective merged images. Scale bar, 5␮m. Images are representative of three to five independent experiments.

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this process does not depend on viral particle assembly but requires the expression of free VP4.

Viruses have developed various strategies to hijack cellular machineries, especially those of the actin cytoskeleton (18). The present study reveals a new way by which a virus protein may interact with microfilaments, as VP4 acts through a simple mecha-nism based on protein-protein interactions and bridging properties. Furthermore, VP4 microfilament remodeling activities do not seem to require any signaling events, contrary to most of the virus-driven actin rearrangements involving Rho GTPase-mediated signaling pathways (35). This conclusion, which is in line with our previously published data (15), reinforces the observation that microfilament remodeling is a progressive process that only depends on the amount of VP4 produced in the target cells and not on the VP4 molecules incorporated in viral particles.

The VP4 peroxisomal targeting signal is functional but masked by an actin-binding domain.As mentioned above, the construct RF-VP4-GFP 718-776 was local-ized in another intracellular compartment that did not colocalize with phalloidin (Fig. 4J to L). Such a type of staining has been already described for VP4 by Mohan et al. (13) and reported to be due to a canonical peroxisome targeting signal (PTS1) in the last FIG 6Homo-oligomerization of free VP4. Cos-7 cells were transiently transfected using plasmids con-taining the construct VP4 481-776, in line with either GFP or mCherry fluorescent proteins. Three days after transfection, Cos-7 cells were observed by confocal microscopy. (A) Typical image obtained with the GFP-containing construct; (B) typical image obtained with the mCherry-containing construct; (C) merged image. Scale bar represents 5␮m. (D) FRET experiment was performed using Cos-7 cells expressing both VP4-GFP and VP4-mCherry (upper) or GFP and mCherry alone (lower). Cells were only illuminated with a 488-nm laser beam, and fluorescence was recovered in the green range (500 to 530 nm) and in the red range (red filter, 570 to 650 nm). Eight different fields were analyzed. Blue color represents the background signal, green color the signal recorded for the GFP channel, and red color the signal for the mCherry channel. In the control lower line, the main signal detected was in the green range, whereas in the upper line a strong red fluorescence was detected in the eight fields analyzed, indicating a FRET signal. Scale bar represents 500␮m. (E) Images from panel D were quantified using ImageJ software, and the ratio of red fluorescence to green fluorescence was calculated for each field. Means and SD were calculated for each experiment and are reported as the percentage of red fluorescence over total fluorescence.

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four C-terminal amino acids of the SA11 rotavirus strain. To explore this further, the reactivity of four VP4 constructs that contained various lengths of its C-terminal part was studied (Fig. 7). For this experiment, the SA11 strain-specific sequence of VP4 was used in order to compare our data with those of Mohan et al. (13). The transfection of the VP4 last four C-terminal amino acids (773 to 776) (Fig. 7A to C and Table 1) coupled with GFP resulted in a pattern that fully colocalized with a peroxisome marker, confirming the functionality of this short domain. The addition of 65 extra C-terminal amino acids (718 to 776) resulted in a pattern where peroxisome colocalization was maintained. However, a filament-like distribution was also clearly visible (Fig. 7D to F). Addition of five more C-terminal amino acids (713 to 776) totally suppressed the peroxisome localization of this VP4 construct, which appeared to be exclusively made of large filament-like structures (Fig. 7G to I). Finally, removal of the four last C-terminal amino acids (718 to 773) abolished the peroxisome colocalization (Fig. 7J to L and Table 1). This demonstrated that VP4 contains an authentic PTS1 that is in competition with another signal that favors interactions with actin microfilaments and suggests that the actin-binding domain dominates over the PTS1 signal. This may explain why peroxi-some colocalization has never been observed during rotavirus infection, even if one considers that VP4 is also expressed as a free protein during the infection process.

Actin binding and remodeling: a common strategy among rotaviruses. The experiments described in Fig. 3, 4, 5, and 7 indicated that VP4 constructs from either the RV or SA11 strains behave similarly regarding interaction with actin microfilaments. Further bioinformatics comparisons of VP4 sequences from several animal strains of species A rotavirus (RRV, RF, SA11, OSU, and chicken), including a human Wa strain, show high conservation, especially within the RV-ABD domain (Fig. 8). Interestingly, even the quite divergent chicken rotavirus, which is characterized by major sequence FIG 7Functional peroxisome-targeting signal is present on VP4 but masked by RV-ABD. Cos-7 cells were transiently transfected using four VP4-GFP (A, D, G, and J) (green) constructs containing (i) the last four amino acids of SA11 strain VP4, namely, QCRL (a typical PTS-1 sequence), termed construct 773-776 (A to C), (ii) the last 59 amino acids of VP4 (construct 718-776) (D to F), (iii) the last 64 amino acids (construct 713-776) (G to I), or (iv) the same construct as construct ii but lacking the PTS signal (construct 718 to 773) (J to L). Three days after transfection, cells were fixed, permeabilized, labeled with Alexa-547-labeled anti-peroxisome antibody (D, E, H, K) (red), and analyzed by confocal fluorescence microscopy. (C, F, I, and L) Respective merged images. Scale bar, 5␮m. Images are representative of three independent experiments.

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variations compared to mammalian strains in species A rotaviruses (36), reveals high conservation in the RV-ABD domain compared to full-length VP4 sequence (Fig. 8). Interestingly, this domain was also found in the available sequences of species B and C rotavirus (Fig. 8). Bioinformatics studies indicated that all of the strains studied displayed a high degree of conservation in the coiled-coil domain. In addition, analysis of the C-terminal sequence of several VP4 proteins from the same strains also showed that the four last amino acids were either identical or similar (Fig. 8), consisting of an authentic PTS1 signal, indicating that rotavirus integrates peroxisome interactions in its strategy, as suggested by Mohan et al. (13). However, as mentioned above, the RV-ABD appears dominant over the PTS1 domain.

This set of data indicates that the ability to bind and remodel actin microfilaments was present in a rotavirus ancestor and was selected a long time ago for an efficient strategy of the virus. Since all these rotavirus strains contained both an actin-binding domain and a coiled-coil domain, it is tempting to hypothesize that VP4 actin binding and remodeling are a common theme for the rotavirus’ replication cycle. However, this conclusion will be difficult to demonstrate as RVC are difficult, and RVB almost impos-sible, to grow in cell culture.

As mentioned above, RV-ABD is located in the C-terminal part of VP4 and is buried when VP4 is assembled within viral particles. To explain how VP4 may still display its capacity to bind and remodel actin microfilaments, we analyzed the only available three-dimensional (3D) structure of VP4 assembled in viral particles (5, 37). Using data from the Protein Data Bank (PDB) (accession number4V7Q), we analyzed the location of RV-ABD in a rotavirus particle. As shown in Fig. 9A and B, RV-ABD was indeed located

FIG 8Sequence alignment of RV-ABD domains from several rotavirus strains. Sequences of VP4 proteins from different strains of species A, B, or C rotavirus were aligned using BLAST. An asterisk indicates amino acid identity, a colon indicates amino acid similarity, a period indicates amino acid divergence (also indicated by black color), and no sign indicates unrelated amino acids. Note that the line below species B and C rotavirus sequences summarizes the comparison of the eight strains studied here. Blue letter codes correspond to the PTS-1 signal. Amino acid numbering was aligned with the numbering of the RF strain.

FIG 9Structural analysis of RV-ABD. (A and B) The 3D structure of a unit of assembled rotavirus particle was retrieved from the PDB (accession number 4V7Q) and is displayed in panel A. The sequence corresponding to RV-ABD was searched, and a zoomed image of this region is displayed in panel B, corresponding to the square in panel A. Mark 1 corresponds to aa 701 (Ile) and mark 2 to aa 776 (Leu) according to RF numbering. Only one of the three VP4 chains (the one annotated BX in PDB) is underlined in black between marks 1 and 2.

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crystalized (32) and functionally studied (39). An integrin-binding domain (40), a fusion domain (11), a coiled-coil domain (40, 41), a dynein-binding domain (42), and a peroxisomal targeting signal (13) have been functionally studied. None of these do-mains accounts for actin-binding and remodeling activity. The present discovery of an original RV-ABD domain and of its likely mechanism of action, together with the demonstration of VP4 expression as a free protein during infection, strongly support the idea that free VP4 plays an important role in rotavirus cellular exit from the apical domain of polarized cells, as shown in our previous studies (6, 14, 15). Interestingly, a recent study (17) proposes that actin is part of rotavirus assembly and exit even in MA104 nonpolarized cells, indicating that cell lysis is not required. These data, together with our previous findings, point again to the general importance of rotavirus-actin interactions in virus morphogenesis and trafficking.

Rotavirus displays a very striking property that consists of the apically polarized release of viral progeny without immediate cell deathin vitro(6) as well asin vivo(43). Since this release process does not involve any conventional vesicular traffic step, one can hypothesize that the apical membrane of intestinal cells has to be transiently destabilized to permit virus exit. Two main factors ensure the high stability of the intestinal brush border membrane, namely its particular “superraft”-based lipid com-position (44) and the highly packed actin microfilament bundles (45). The present results provide a molecular mechanism for VP4-driven brush border membrane desta-bilization through its actin microfilament remodeling activity. Interestingly, it has been shown that another viral protein, namely, NSP4, may also disturb the actin cytoskeleton and, although not colocalizing with F-actin, causes F-actin reorganization (46). These authors have shown that, in NSP4-transfected cells, actin reorganization occurs in a calcium-dependent manner, causing an increase in F-actin content and a decrease in phosphocofilin, the inactive form of cofilin. These data are in line with the present work and point to a coordinated action of viral NSP4 and VP4 proteins. By increasing intracellular calcium levels, NSP4 activates cofilin, resulting in the generation of addi-tional barbed ends, which are the targets of VP4 (15). In the cells thus prepared for VP4 action, the spike protein would apply its microfilament bridging activity. The most striking feature of this viral strategy is that neither the action of NSP4 (46, 47) nor that of VP4 (15) is dependent on the Rho GTPases involved in the physiological regulation of F-actin network dynamics and represents a novel mode of action for a virus.

MATERIALS AND METHODS

Cells infection and transfections. MA104 cells were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Invitrogen, UK), 100 U/ml penicillin, and 100␮g/ml of streptomycin (all reagents were from Life Technologies, Saint-Aubin, France) at 37°C with 5% CO2.

Production and titration of the bovine rotavirus RF strain were carried out as previously described (14, 15). Infections of cells were performed at a multiplicity of infection of 10 PFU per cell, as previously described (14, 15). Cos-7 monkey kidney cell lines were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum, 100 U penicillin, and 0.1 mg streptomycin/ml (Invitrogen, Cergy-Pontoise, France). Transfections were realized using Exgen500 (Euromedex, Souffelweyersheim, France) or Effectene (Qiagen, Courtaboeuf, France). Briefly, 24 h after transfection, cells were trypsinized and seeded on glass. Experiments were carried out 24 to 48 h posttransfection.

Plasmid construction.The full-length VP4 (of bovine rotavirus RF strain) cDNA (GenBank accession numberU65924) was a gift of Jean Cohen (CNRS, Gif-sur-Yvette, France). Unless stated otherwise, VP4 was from the RF strain. VP4 (SA11 strain) cDNA was kindly provided by Satoshi Komoto and Koki

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Taniguchi (Department of Virology and Parasitology, School of Medicine, Fujita Health University, Japan). Plasmid bearing Sp100 cDNA was kindly provided by Hugues de Thé (CNRS U944/INSERM UMR7212, Paris, France). All constructs encoding GFP-truncated VP4 were made using the backbone plasmid pEGFP-C1 (Clontech, Ozyme, Montigny le Bretonneux, France). Truncated VP4 constructs encoding stretches of aa 481 to 776, 481 to 773, 481 to 757, 562 to 776, 641 to 776, 641 to 773, 641 to 757, 702 to 776, 713 to 776, 713 to 773, and 718 to 776 were generated by PCR and inserted within the pEGFP-C1 vector via EcoRI and BamHI sites. Arbitrarily, each GFP-truncated VP4 construct was designated by the limits of amino acid sequences preceding VP4; e.g., VP4 with full-length GFP (aa 1 to 776) is designated VP4 (1-776). Insertion of VP4 coiled-coil segment VP4 (481-574) in VP4 (713-776) was made in the EcoRI site. Sp100 coiled-coil segment fused to VP4 (562-776) was made by insertion of PCR-amplified Sp100 coiled-coil aa 129 to 182 in VP4 (562-776). GFP-truncated SA11 VP4, encoding amino acid stretches 713 to 776, 718 to 776, 718 to 773, and 733 to 776, were made by insertion of amplified segments in pEGFP-C1 via XhoI and BamHI sites. For PCR experiments, the following primers were used: 481RF forward, 5=-CTTCGAATTCTACACCGAAACTGTATGGAGTG-3=; 562RF forward, 5=-CTTCGAATTCTTCAACGCT CACTGATTCATTGTCTG-3=; 641RF forward, 5=-CTTCGAATTCTTCAACGCTCACTGATTCATTGTCTG-3=; 702RF forward, 5=-CTTCGAATTCTCCATTTGATGTACAAAAATTCGCTG-3=; 713RF forward, 5=-CTTCGAATTCTACTG ACTCACCAGTTATATCGGCAA-3=; 718RF forward, 5=-CTTCGAATTCTATATCGGCAATAATTGACTTTAAAAC-3=; 757RF reverse, 5=-GAGGTGGATCCTTAATTAATAAATTCACGTAATAG-3=; 773RF reverse, 5=-GAGGTGGATCCT TATTGCATTATCAAACTTTC-3=; 776RF reverse, 5=-GAGGTGGATCCTTACAAGCGACATTGCATTATC-3=.

The coiled-coil fragment of Sp100 was PCR amplified using the following primers: Sp100CC forward, 5=-CTTCGGAATCCTGTCAACATGCAGGAATACCCCGA-3=; Sp100CC reverse, 5=-AACTACTTAAG AGCTTTTCAAAAGTGGTC-3=. For FRET experiments, an additional 481-776 construct was inserted in an mCherry plasmid via EcoRI and BamHI sites.

Immunofluorescence and FRET experiments. Cells were cultured on 12-mm-diameter circular coverslips. For immunofluorescence experiments, cells were washed three times in phosphate-buffered saline (PBS; GIBCO) and fixed with 4% paraformaldehyde for 20 min at room temperature. Permeabili-zation was performed in PBS– 0.2% Triton X-100 for 10 min. MAbs used (7.7 and 2G4) were generous gifts of Jean Cohen and Mary Estes. Secondary antibodies were anti-mouse Alexa Fluor 488 antibody (Molecular Probes, USA) and anti-mouse Cy3-conjugated antibody (Jackson Immunoresearch lab.). For actin staining, samples were incubated with Alexa-547-phalloidin (FluoProbes, Montluçon, France) in PBS– 0.1% Tween 20 for 2 h at room temperature. Peroxisomes were localized using a PTS1-dsRed probe (Clontech, Saint-Germain-en-Laye, France). After extensive washes, cells stained with 4= ,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich, Lyon, France) for 3 min were treated for 10 min with 100-mg/ml 1,4-diazabicyclo-[2.2.2]-octane antifading reagent (Sigma-Aldrich, Lyon, France) and mounted with Fluoprep (bioMérieux, France). Observations were made by a confocal microscopy LSM 510 META with a Zeiss 63⫻ (numeric aperture, 1.4) oil immersion lens, and data acquisition was performed sequentially for each channel. Confocal microscopy images were deconvoluted with AutoDeblur Soft-ware (AutoQuant Imaging Inc., Troy, NY). Images were mounted using Photoshop in .tiff format.

For FRET experiments, Cos-7 cells were transiently transfected with either VP4 (481-776)-GFP to-gether with VP4 (481-776)-mCherry or with control plasmids containing only GFP and mCherry. Mea-surements were performed on the same microscope, using the 488-nm laser line. Emitted fluorescence was recovered in eight different fields by adjusting the emission windows at 500 to 530 nm to collect green fluorescence or at 570 to 650 nm to collect the red fluorescence. The two channels were collected sequentially to avoid any interference. Fluorescence intensity in each channel was measured using ImageJ software. The red/green fluorescence ratio was then calculated for each field. Means and standard deviations (SD) were calculated for each experiment.

Protein sequence analysis.Sequences of VP4 proteins from different strains of species A rotavirus (Wa, GenBank accession numberKT694942.1; OSU, GenBank accession numberKJ450845.1; RF, GenBank accession numberKF729690.1; RRV, GenBank accession numberAY033150.1; SA11, GenBank accession number LC178567.1; chicken, GenBank accession number ACN22281.1), species B rotavirus (IDIR, GenBank accession number X16949), or species C rotavirus (Shintoku, GenBank accession number

AB738415.1) were aligned using BLAST software (NCBI). Coiled-coil domain prediction was made using the tool available online from PRABI (Pole Rhone-Alpes de Bioinformatique;https://npsa-prabi.ibcp.fr/ cgi-bin/npsa_automat.pl?page⫽npsa_lupas.html).

Structural prediction.Structural models were constructed using different prediction tools and web servers that are freely available (SAM-T08 [48], Robetta [49], I-Tasser [50], HHPred [51], Phyre [52], PCons [53], and LocPred [54]). Models were further ranked using cutting-edge evaluation scores, Qmean (55), ModFold (56), or Pcons (53). Final selected models result from a compromise between the evaluation scores and the structural agreement of the models obtained for rotavirus from species A (RF and SA11) and species C (Shintoku) sequences. Models for the 3D structure of VP4 embedded within viral particles were retrieved from PDB (accession number4V7Q). The sequence of RV-ABD was directly identified on the 3D model. Only one of the three VP4 chains (the one denoted BX in PDB4V7Q) present on the model was underlined.

ACKNOWLEDGMENTS

This work was supported by institutional funding of INSERM, University Pierre et Marie Curie (UPMC). W.C. is a recipient of a postdoc grant from ANR (ANR ProbDOM). We thank Philippe Fontanges and Romain Morichon for image production at the Imaging Platform of Centre de Recherches Saint-Antoine (INSERM-UPMC, Paris, France).

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