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Highly Purified Human Immunodeficiency Virus Type 1 Reveals a Virtual Absence of Vif in Virions

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FIG. 1. Protein expression pattern in HIV-1-infected and Vif-expressing celllines. Cell lysates from mock-infected, HXB2NEO, and H9(LVifSN) cell lines
FIG. 2. Sucrose density-equilibrium gradient analysis of extracellular supernatants from HXB2NEO and H9(LVifSN) cell lines
FIG. 3. Comparison of sedimentation patterns of extracellular nonviral andviral proteins by sucrose density-equilibrium gradient or OptiPrep gradient anal-
FIG. 4. OptiPrep gradient analysis of extracellular Vif from HXB2NEO H9cells. Extracellular supernatants were pelleted through a 20% sucrose cushion
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