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Hepatitis B virus large surface protein is not secreted but is immunogenic when selectively expressed by recombinant vaccinia virus.

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FIG. 1.BamHI;sequencessequences. Construction of recombinant plasmid vector. The entire HBV surface antigen coding region sl-s2-s was reconstructed from HBV contained in pGS27 and pAM6 and placed downstream of the vaccinia virus promoter P7.5 and flanked b
TABLE 1. RIA of s and pre-s antigen particles
FIG. 3.againstthemarkersinfectedingThevirussolubilized Immunoblot of LS and S incubated with antibody raised synthetic peptide corresponding to amino acids 13-40 from NH2 terminus of LS
FIG. 5.or pre-s2 Induction of antibody to pre-s epitopes. RIAs were performed in microtiter wells coated with peptides from the pre-sl (panel A) (panel B) regions, as described in the text.
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