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Recombinant respiratory syncytial virus from which the entire SH gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse.

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Academic year: 2019

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Figure

FIG. 1. Diagrams (not to scale) of the parental wild-type D46 plasmid encoding an RSV antigenome (top) and the D46/6368 derivative in which the SH gene hasbeen deleted (bottom)
FIG. 2. Comparison of D46/6368 SH-minus and D46 wild-type recombinant RSV with regard to the ability to form plaques (A) and syncytia (B) in monolayers ofHEp-2 cells
FIG. 3. RT-PCR analysis of total intracellular RNA from cells infected withthe D46 wild-type or D46/6368 SH-minus virus to confirm the deletion in the SH
FIG. 4. Northern blot hybridization of RNAs encoded by the D46 wild-type and D46/6368 SH-minus viruses
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