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Assessment of drug induced genotoxicity in mammalian cells and the contribution of topoisomerase II inhibition

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Figure

Figure 1.1: The Bacterial Reverse Mutation Test Schematic of testing regime for a two or 5 strain Ames test
Figure 1.2: Metaphase Preparation of Human Lymphocytes for analysis of
Figure 1.3:  The Microtitre Mouse Lymphoma Assay 3 hour incubation use as standard, 24 hour incubation used to identify division dependent mutagens, cells cultured for 2 days post-treatment then plated in selective (TFT containing) or non-selective medium TFT = trifluorothymidine, selectively kills non-mutant (heterozygous tk+/- cells)
Figure 1.6:  The Rodent Haemopoietic Cell in vivoRodents dosed daily twice then sacrifice 24 hours after final dose, bone marrow aspirated from femurs, resultant cells prepared on microscope slides, fixed and DNA stained with acridine orange prior to micronuclei determination  Micronucleus Test
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