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Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate

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Figure

FIG. 1. Effect of TGEV nsp1 on host protein synthesis. (A) HEK293 cells (left) or ST cells (right) were cotransfected with plasmid pRL-SV40encoding the rLuc reporter gene downstream of the SV40 promoter and one of the following plasmids: pCAGGS-CAT (CAT), pCAGGS-TGEV
FIG. 3. Analyses of TGEV nsp1-induced translational suppression in vitroHeLa S10 extract for 30 min in the presence of 1of the rLuc gene and CrPV IRES-driven translation of fLuc were measured by the dual luciferase assay kit (Promega)
FIG. 4. Characterization of TGEV nsp1-induced translational sup-pression. (A) 293 cells were independently transfected with capped

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