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The Viral Protein Tat Can Inhibit the Establishment of HIV-1 Latency

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Figure

FIG 1 Model of HIV-1 latency establishment and reactivation. Jurkat cells were infected with NL4-3-�E-EGFP or the attenuated tat (H13L) derivative andcultured for up to 8 weeks
FIG 2 Biological activity of recombinant Tat proteins. Purified T23N (increased transactivation variant) and C22G (transactivation-negative) Tat proteins wereadded to the LTR/Tat-dependent reporter cell lines JLTRG-R5 (A and B) or Tzm-bl (C), in the presenc
FIG 4 Jurkat and Jurkat-tracted from total infection). Results represent meanviral EGFP was measured by flow cytometry 24 h later.compare total (activetat cells are equally susceptible to viral infection
FIG 5 Potent inhibition of the establishment of latency by intracellularly expressed Tat
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