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The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion

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Academic year: 2019

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Figure

FIG 1 NS1 inhibits NLRP3 inflammasome-mediated IL-1� secretion. (A) HEK293T cells were transfected with expression plasmids encoding either NLRP3,ASC, procaspase-1, pro-IL-1�, and human UCP-2 (hUCP-2) or NS1
FIG 2 Influenza virus lacking the NS1 gene failed to stimulate IL-1infected with wild-type (WT) or recombinant influenza virus lacking the NS1 gene (protein expression was analyzed by immunoblotting
FIG 4 NS1 protein interacts with NLRP3. (A) HEK293T cells were cotransfected with expression plasmids encoding Flag-tagged NLRP3, ASC, or RIG-I togetherwith myc-tagged NS1
FIG 6 The NS1 protein from different strains suppresses NLRP3 inflammasome-mediated IL-1plasmids encoding NLRP3, ASC, procaspase-1, pro-IL-1supernatants were collected at 24 h posttransfection, and IL-1were collected at 24 h posttransfection, and NLRP3 expr
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