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Confocal laser scanning microscopy analysis of S epidermidis biofilms exposed to farnesol, vancomycin and rifampicin

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Figure

Figure 1 Effect of farnesol, vancomycin, and rifampicin on viability of S. epidermidis biofilm cells versus time of treatment.* statisticallydifferent as compared with the control, at the respective time-point (p < 0.05).
Figure 2 Effect of farnesol and antibiotics on biofilm structure and viability. (A) Biofilm without antimicrobial agent; (B) Negative controlfor live/dead staining, treated with 96% ethanol for 4 h; (C) Biofilm exposed to 300 μM farnesol for 24 h; (D) Biofilm exposed to 40 mg/L ofvancomycin for 24 h; (E) Biofilm exposed to 10 mg/L of rifampicin for 24 h.
Figure 3 Effect of farnesol and antibiotics on biofilms maximum depth. * statistically different as compared with the control (p < 0.05).

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