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Selective decrease in the rate of cleavage of an intracellular precursor to Rauscher leukemia virus p30 by treatment of infected cells with actinomycin D.

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Academic year: 2019

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Figure

FIG.1.similarlycontainingcontainingwere75 min Effect ofAMD on viral RNA and protein synthesis
FIG. 2.proteins,pulseforoflinearwas(C),AMD Effect ofAMD on the cleavage ofPr4 to p30. RLV-infected JLS-V16 cells were pretreated with 5 pg per ml for 1 h in complete growth medium.-The culture fluid was removed, and the cultures were labeled for 15 min wit
FIG. 3.15completemin;with min Effect of cycloheximide on the cleavage ofPr4 to p30. RLV-infected JLS-V16 cells were pulsed for with [35S]methionine in Hanks buffer (A) and chased for the following times: (B, C) 15 min; (D, E) 30 (F, G) 60 min; (H, I) 3 h
FIG. 5.200AMDforcellsinpg the Combined effect ofAMD and cycloheximide on the extent ofcleavage ofPr4 to p3O
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