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Ectromelia virus RING finger protein is localized in virus factories and is required for virus replication in macrophages.

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TABLE 5. In vitro multiplication of EV and the p28� mutantin peritoneal macrophages from resistant (B6) mice
FIG. 1. Viral DNA synthesis in EV or p28�rophages. Total DNA was extracted from triplicate cultures at 0, 8, and 24 h afterinfection with EV or the p28hybridization of total infected cell DNA with a mutant-infected peritoneal mac-� mutant, and viral DNA wa
Fig. 2E with F). These results appeared to rule out the possi-bilities that the observed effects were due to a small subpopu-lation of cells other than macrophages, that the E3L gene was
FIG. 5. Subcellular localization of p28 in EV-infected macrophages. Indirect immunofluorescence analysis of p28�with anti-p28 antiserum at 16 h after infection
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