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The Papain-Like Protease of Severe Acute Respiratory Syndrome Coronavirus Has Deubiquitinating Activity

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Figure

FIG. 1. Defining a core SARS-CoVPLpro domain using a transprecipitated with antibody anti-R1, which precipitates the uncleavedprecursor NSP1-3* and cleavage product nsp1
FIG. 3. Identifying residues essential for proteolytic activity of SARS-CoV PLpro. SARS-CoV PLpro constructs encoding the wild-typesequence or specific alanine substitutions were coexpressed with substrate NSP1-3* as described in the legend of Fig
TABLE 1. Apparent kcat/Km (kapp) values for SARS-CoV PLprowild type and mutants

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