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A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping

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Figure

FIG. 1. Reconstitution of VSV mRNA synthesis in vitro. (A) Virus(V), ribonucleoprotein cores (R), N RNA template (T), and BV-
FIG. 2. Alignment of CRV of the L protein of NNS RNA viruses. The alignment was generated using CLUSTALW, and conserved residueswere emphasized by BOXSHADE
FIG. 5. Substitutions in CRV of L protein result in defects in mRNA cap formation. (A) Schematic of the 3�products of in vitro RNA synthesis
FIG. 7. Defects in cap formation are independent of defects in processivity. (A) Five nucleotides of RNA corresponding to the 5�or mRNA was synthesized by T7 RNA polymerase and purified by 20% PAGE
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