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Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates

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Figure

Figure 1. Conformational changes on the double-flap DNA structure induced by the addition of Mgstructure induced by Mgdistance (Atwo-state model described by Supplementary Equation S1 (see Supplementary ‘Materials and Methods’ section) are shown as continuo
Figure 2. Structural transitions in the Flap-23 substrate studied by sm-FRET. (molecules in the absence (panel A) and presence of increasing Mgcontributions from fluorophore blinking and photobleaching
Figure 3. Organization of the ternary complex studied by FRET and PIFE. (complex using MD simulation derived from (26) and the FRET distances extracted in (b)
Figure 4. DNA distortion by Fen1 in the absence (squares) and presence (circles) of PCNA at 10 mM Camolecular FRET was used to monitor the interaction between Fen1 and different flap substrates (represent the fitting to the FRET-binding isotherm for each flap
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