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Molecular Determinants for Subcellular Localization of the Severe Acute Respiratory Syndrome Coronavirus Open Reading Frame 3b Protein

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Figure

TABLE 1. Oligonucleotides used for cloning and site-directed mutagenesis
FIG. 1. Confocal analysis of the intracellular localization of the SARS-CoV ORF 3b. (A) Vero cells were transfected with ORF 3b-EGFP,counterstained with MitoTracker (red) or Hoechst (blue) to identify mitochondria and nuclei, respectively, and observed by confocal microscopy.
FIG. 2. FRAP analysis of nuclear and mitochondrial ORF 3b-EGFP. Vero cells were transfected with ORF 3b-EGFP, and cells expressingnuclear ORF 3b (A) or mitochondrial ORF 3b (B) were selected for analysis
FIG. 3. Intracellular targeting of ORF 3b-EGFP truncations. (A) Schematic representation of C-terminal EGFP constructs used to determinethe mitochondrial localization sequence
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