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Implication of Serine Residues 271, 273, and 275 in the Human Immunodeficiency Virus Type 1 Cofactor Activity of Lens Epithelium-Derived Growth Factor/p75

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Figure

FIG. 1. (a) Salt extraction assay. TL3 cells expressing the LEDGF/p75 WT or WT-FLAG were lysed in a buffer containing increasing NaClconcentrations
FIG. 4. HIV-1 DNA integration in TLEDGF/p75 S271A/S273A/S275A mutant. Results from two indepen-dent experiments using different viral preparations are represented.DNA products were quantified by real-time PCR 10 days after infec-tion
FIG. 6. Interaction of LEDGF/p75 mutants with HIV-1 integrase.(a) Integrase protection assay
FIG. 7. Integrase-to-chromatin-tethering assay. LEDGF/p75-deficient HEK293T cells stably expressing EGFP-tagged HIV-1 integrase wereeither transiently transfected or not transfected with plasmids expressing the LEDGF/p75 WT or mutants and analyzed by fluorescence

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