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Hepatitis C Virus Core Protein Is a Dimeric Alpha-Helical Protein Exhibiting Membrane Protein Features

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Figure

FIG. 1. Sequence analysis of HCV core protein and comparison with GBV-B core protein. (A) alignment of the amino acid sequences of theHCV and GBV-B core proteins
FIG. 2. Expression and purification of CHCVspond to proteins purified by Ni-NTA affinity chromatography.Cduced (lanes 1 and 5) and induced (lanes 2 and 6) BL21(DE3)/SI cellsexpressing CE
FIG. 3. Sedimentation velocity of CHCVof differences between the experimental and fitted profiles
FIG. 4. Equilibrium sedimentation of CHCVcorresponding panels. The equilibrium sedimentation profiles were recorded for three rotor velocities (13,000, 16,000, and 22,000 rpm from topto bottom at the origin)
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