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Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication

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Figure

FIG. 1. Schematic diagrams of the chimeric PV genome encoding the N-terminal amphipathic helix in protein 2C from HRV14 and pseudo-revertants
TABLE 1. Large plaque viral isolates with mutationsoutside of the 2C-encoding region
FIG. 3. Accumulation of chimeric PV RNAs in transfected cells. (A) HeLa cell monolayers were transfected with 1 �described previously (31)
FIG. 5. Mammalian cell two-hybrid analysis of interactions of PV fusion proteins. COS cells were cotransfected with pBind-2C (A and B) orpBind-2BC (C and D), and different pAct plasmids that drive expression of 2B, 2C, 2BC, 3A, and 3AB fusion proteins and the reporter vector
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