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Assessment of a DNA vaccine encoding Burkholderia pseudomallei bacterioferritin

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Figure

Figure 1:  Construction of the bacterioferritin DNA vaccine Bps190. The bacterioferritin gene was amplified by PCR and cloned into the intermediate vector pCRII TOPO to ensure alignment and a correct reading frame
Figure 2:  In vitro expression of bacterioferritin encoded in the DNA vaccine detected by western blot
Figure 3:  Survival of mice following challenge with live B. pseudomallei NCTC 13179. Ten mice immunised with either the pBps190 or pcDNA3.1start alone (sham) received 2xLD50 IP of live B
Figure 4:  Bacterial loads in spleens of mice following DNA vaccination and challenge with live B
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