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The Flexible Loop of the Human Cytomegalovirus DNA Polymerase Processivity Factor ppUL44 Is Required for Efficient DNA Binding and Replication in Cells

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Figure

FIG. 1. The highly conserved flexible loop (residues 162 to 174) within ppUL44 protrudes from ppUL44 basic face and is important for efficientnuclear accumulation and localization in nuclear speckles
FIG. 2. UL44-FL is required for ppUL44 intranuclear binding as determined by FRAP analysis
FIG. 3. GFP-UL44 associates with dsDNA and the nuclear matrix, in contrast to GFP-UL44L86A/L87A and GFP-UL44�followed by incubation with NaCl 2 M for 5 min (DNase I, NaCl)
FIG. 4. ppUL44 dimerization and UL44-FL are required for invivo DNA binding. (A) HEK 293 cells expressing the indicated fusion
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