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The Cellular DNA Helicase ChlR1 Regulates Chromatin and Nuclear Matrix Attachment of the Human Papillomavirus 16 E2 Protein and High-Copy-Number Viral Genome Establishment

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Figure

FIG 1 HPV16 E2 associates with the N terminus of ChlR1. (A) The His-tagged ChlR1 1-130 and 63-214peptides were expressed and purified from E
FIG 2 Isolation of a ChlR1-binding-defective mutant of HPV16 E2. (A) Overlay of the N-terminal transactivation domains ofcells growing in 10-cm dishes were transfected with 2for coimmunoprecipitation reactions are shown in the left panels
TABLE 1 Analysis of ChlR1 binding of HPV16 E2 mutants in comparison to E2WT
FIG 3 HPV16 E2or in combination with increasing amounts of an E2Y131A is transcription and replication competent
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