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Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system.

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FIG. 1. Schematic of the HIV-1NL4-3(31). Oligonucleotide primers used in the cell-free system (indicated by arrowheads; not to scale) are positioned at their approximate locations along the RNA templates(black horizontal bars at the bottom)
FIG. 3. Cell-free minus-strand DNA synthesis. Single-stranded RNAs from pHIV-nefReaction times were varied from 0 to 120 min, products were resolved by urea PAGE, and the resulting autoradiographs are shown
FIG. 4. Cell-free plus-strand DNA synthesis from the 3�the 20-mer primers were not radioactively labeled
FIG. 5. Cell-free plus-strand synthesis from non-PPT sites. DNA synthesisreactions were carried out for 40 min as described in Materials and Methods and
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