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Producer-cell modification of human immunodeficiency virus type 1: Nef is a virion protein.

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Figure

FIG. 1. (A) Schematic of the LTR-nefApproximately 10Nef. (B) Western blot of parental CEM cells and CLN cells infected with either�analyzed for expression of Nef protein by Western blotting as described inintroduced into CEM cells to create the cell line C
TABLE 1. Infectivities of wild-type and nef mutant viruses assessedby syncytium formation on HeLa-T4 cells
FIG. 7. HIV-1 protease cleaves virion-associated Nef protein. Shown is aWestern blot of purified virion preparations generated by transient transfection.

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