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Editing the genome of Aphanomyces invadans using CRISPR/Cas9

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Figure

Table 1 PCR primers designed for each guide RNA. Three PCR primer sets corresponding to each gRNA were designed based onthe A
Fig. 3 PCR result (214 bp) of RNP-2-treated and non-treatedprotoplasts and zoospores using primer set 2
Fig. 5 Dwarf gourami showing necrotic ulceration after infection with the zoospores ofNegative control fish at 7 dpi.ulceration (arrow) at the site of infection at 7 dpi.and the beginning of ulcer formation (arrow) at the site of infection at 3 dpi.10,000
Fig. 7 Hematoxylin and eosin-stained muscle sections of dwarfgourami. a Muscle section of positive control fish showing musclenecrosis (arrow)

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