Intracellular Virus-Specific Structures and RNAs in Oncornavirus-Producing Human Cells

As a control to ensure that phosphorylation of UL37 in the V37-infected cells was protein specific, 32P-labeled V8- infected-cell protein extracts were also immunoprecipitated with

The cells were labeled from 5 to 18 h after infection with [3H]thymidine, and then extraction, sucrose gradient purification, sample deproteinization, and CsCl centrifugation

RNA-directed DNA polymerase activity in the cytoplasmic fraction of REV-T transformed chicken bone marrow cells resolved by sedimentation velocity sucrose gradient

Buoyant density distribution in CsCl equilibrium density gradients of DNA extracted at 9 h postinfection from the nuclei ofHEp-2 cells infected with 32P-labeled HSV-I (F) virus..

Reassociation kinetics of 32P-labeled SV40 DNA incubated in the presence of unlabeled DNAs extracted from Hirt supernatant and pellet fractions of mitomycin C-induced and control

inhibited equally well hybrid formation be- It will therefore be interesting to investigate in tween labeled cytoplasmic 3H-RNA and cellular detail the transport mechanism of viral

Counts per minute of 3H-thymidine incorporated into acid-precipitable material extracted from nuclear and cytoplasmic fractions of 6- and 10-hr infected cells and from uninfected

rose gradient centrifugation of cytoplasmic extract ob- tained I hr after cell infection with Sendai virus labeled simultaneously with 3"PO4 and 3H-leucine. Cells (2 X 107/ml)