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A Strain-Specific Epitope of Enterovirus 71 Identified by Cryo-Electron Microscopy of the Complex with Fab from Neutralizing Antibody

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FIG 1 Cryo-EM micrographs of data and the resolution of reconstructions. (A and B) Cryo-EM data sets of Fab28-7 incubated in excess with EV71 in aFab/capsid ratio of 240:1 and 24:1 for data sets 1 (A) and 2 (B), respectively
TABLE 2 Characterization of EV71 strains
FIG 3 Asymmetric reconstruction and fitted Fab structure. (A) An asymmetric approach aimed at reconstructing one Fab fragment bound to the capsidsurface resulted in strong Fab density at �30% of the magnitude of the capsid density
FIG 6 Sequence alignments of the 17 EV71 virus strains used in Chen et al. (IIb 12 MAbs, whereas strains in the blue box (antigenic types 2.3 and 2.4) are not neutralized by the same class

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