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Ultrasensitive single genome sequencing: accurate, targeted, next generation sequencing of HIV 1 RNA

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Figure

Fig. 1 Schematic representation of the methods used for NGS library construction. A cDNA library labeled with Primer IDs (top) is divided and used for each method
Table 1 Comparison of cDNA amplification efficiency among methods using the same HIV-1 site specific RT-Primer ID primer
Table 2 Comparison of Recombination between methods at different consensus majority cutoffs in mixtures of BH10 WT and mutant transcript RNA
Fig. 2 Neighbor joining trees comparing PCR recombination in each method. Neighbor joining trees rooted on NL4-3 generated from randomly selected sets of 50 supermajority sequences obtained from mixtures of WT and Mutant BH10 pol transcripts
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