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Metabolic biomarkers for response to PI3K inhibition in basal like breast cancer

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Figure

Figure 1 pAktpan-Akt (green) antibodies, and secondary antibodies conjugated with near-infrared (NIR) fluorescent dyes (red channel, pAktand phosphatase and tensin homolog (PTEN) in lysates from two different BL and LL cancers
Figure 2 Three-day treatment of drugs targeting phosphatidylinositol 3-kinase pathway reduces active Akt level in basal-like(B)treatment with MK-2206 and BEZ235 in BL but not in LL xenografts
Figure 3 Plasma membrane-associated pAkt4The sections were first imaged with a near-infrared (NIR) scanner and subsequently imaged by confocal microscopy to detect the fluorescent-labeledantibodies in the visible light area
Figure 4 pAktquantified both in the NT and in the CT. Quantifications were done in three to five randomly selected circular regions of interest of the tumors andpresented as the mean relative intensity in the different areas with standard deviations.basal-
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