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OrfA Downregulates Feline Immunodeficiency Virus Primary Receptor CD134 on the Host Cell Surface and Is Important in Viral Infection

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Figure

FIG. 1. CrFK, GFox, and 104-C1 cell infection assay using FIV-34TF10 and OrfArep strains
FIG. 2. FACS assays for detection of cell surface receptors. (A) Detection of CD134 and CD9 receptors on 104-C1 and 104-C1-OrfA cells.(Left) Detection of CD134 using rabbit anti-CD134 antibody
FIG. 3. OrfA mRNA quantitation and FACS assays on CD134surface levels for siRNA-treated OrfA knockdown cells
FIG. 7. Confocal microscopy analysis showing CD134 subcellular localization in 104-C1 and 104-C1-OrfA cells

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